CRISPR-edited Immortalized Knockout Cells
CRISPR knockout immortalized cell lines — pools or clones with guaranteed editing efficiency, optimized across more than 500 human cell lines and delivered assay-ready.
Fast, Reliable CRISPR Knockout Pools and Clones
Consistent knockouts you can trust — XDel multi-guide designs with 98% average editing efficiency, NGS-verified, assay-ready in as fast as 2 weeks.Knockouts you can trust
Consistent, reproducible knockouts with 98% average editing efficiency;
Assay-ready on arrival
Pools shipped in as fast as 2 weeks, clones as fast as 8 weeks;
No guesswork
NGS-verified with a QC report on every order;
Unmatched CRISPR Specialty
Experience with 1400+ cell lines and 650k+ CRISPR edits.
EditCo’s Automated Immortalized Cell Line Editing Process
200 Ways We Optimize CRISPR to Achieve High Editing Across Cell Lines
EditCo’s 200-point optimization process has been performed across more than 500 human cell lines spanning 15 tissue types, identifying the optimal editing conditions for each cell line. Over 400 cell lines achieve knockout efficiencies of 75% or higher.
Our gene editing workflow includes the use of our unique XDel Technology or single-guide transfection, genotyping, and sequencing analysis. Choose from either EditCo supplied cell lines banked from our high-quality cell supplier ATCC, or onboard your cells.
Knockout Cell Libraries
Everything you need to get screening with Speed and Confidence
EditCo’s Engineered Cell Libraries simplify high-throughput CRISPR screening for functional assays, overcoming traditional barriers like infrastructure limitations and transfection challenges.
These custom panels of knockout immortalized pools, designed using advanced multi-guide technology, achieve editing efficiencies over 90% and are provided in a ready-to-use 96-well arrayed format. With streamlined workflows, comprehensive QC reports, and compatibility with diverse downstream assays, our libraries empower you to confidently discover and validate drug targets at scale—letting you focus on your assay while we handle the CRISPR complexities.
Learn more about our Knockout iPSCs, also available as cell libraries.
Features
| Cell Source |
|
|---|---|
| Available Edits | Single knockout cell pools |
| CRISPR Design | Multi-guide synthetic modified sgRNA (standard) |
Deliverables
- Regular updates on your order's progress
- User-specified knockout cell pools per library (2 vials of each pool with ~20,000 cells/tube, 20 minimum order)
- 5 control pools per rack: 2 mock WT, 2 multi-guide KO, 1 single-guide KO
- Sequence of synthetic sgRNA used
- Primer sequences used for PCR and Sanger Sequencing
- ICE Sanger sequencing analysis reports
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Engineered Cell Libraries Demonstrate High Editing Efficiencies Across Cell Types
Engineered Cell Libraries provide a set of CRISPR-edited cell pools that can be used directly in functional assays for a wide array of different screening and discovery applications. Editing efficiencies for each pool are assessed by sequencing and ICE analysis to inform the interpretation of assay results.
Knockouts in Human Immortalized Cell Line
Figure 10. A library of knockout cell pools corresponding to 42 gene targets was generated via high-throughput editing in human cells. Data from the experiment are shown above. An Engineered Cell Library with 42 gene targets was generated via high-throughput editing of an immortalized-adherent human cell line, A375, using multi-guide technology. The data demonstrate both indel frequencies and Knockout Scores. Of the 84 percent of pools successfully sequenced and analyzed, an average indel frequency of 99 percent and an average Knockout Score of 98 percent were observed.
Knockouts in Mouse Immortalized Cell Line
Figure 11. A library of knockout cell pools corresponding to 192 gene targets was generated via high-throughput editing in mouse cells. The data demonstrate both indel frequencies and Knockout Scores. Among the 80 percent of pools successfully analyzed, the average indel frequency was 89 percent, while the average Knockout Score was 84 percent.
Our Unique Guide Design Powers Functional Knockouts and Sustained Protein Depletion
The multi-guide approach consists of up to 3 gRNAs (purple bars) spatially coordinated that target a single early exon of a gene and induce multiple concurrent double-strand breaks in the target sequence. This results in disruptive one or more >21 bp fragment deletions. This approach is associated with higher frequencies of knockout alleles in edited pools but reduced occurrence of off-target edits as compared to single-guide approaches.
Figure 12. The multi-guide approach consists of 3 gRNAs spatially coordinated to create a fragment deletion in a single exon. Targeting a single early exon of a gene induces multiple concurrent double-strand breaks in the target sequence of a gene. This results in disruptive one or more >21 bp fragment deletions.
Figure 13. Edited cell pools utilizing the multi-guide technology shows persistent protein depletion. This enables direct and reliable use of these cell pools for functional assays. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.
Testimonial
Simon LebaronPhD-HDR, CRCN INSERM. Université de Toulouse (CBI, MCD Unit), France.“As a researcher studying the newly characterized factor SURF2 and its role in cancer resistance to chemotherapies, we aimed to investigate the effects of its depletion on cell proliferation and phenotypic traits using U2OS and HepG2 cell lines. While siRNA strategies initially yielded promising results, we sought a more robust and long-term solution to avoid potential biases caused by transfection-induced stress responses.
Lacking the time and expertise to develop a CRISPR strategy within our team, we turned to EditCo for assistance in generating knockout pools for these cell lines. Within a remarkably short time, we received pools with 100% homozygous deletions. After verifying the results ourselves, we were impressed by the efficiency and quality, even isolating clones to further refine our analyses.
This allowed us to rapidly assess the impact of SURF2 knockout on key cellular parameters, culminating in the publication of our findings in Nature Communications (Tagnères et al.). These cell lines continue to play a pivotal role in advancing our research on SURF2, and we wouldn’t hesitate to collaborate with EditCo again for future CRISPR projects. Their expertise and efficiency have been invaluable to our work.”
Read more in the Nature Communications paper
SURF2 is a MDM2 antagonist in triggering the nucleolar stress response
Tagnères, S., Santo, P.E., Radermecker, J. et al. SURF2 is a MDM2 antagonist in triggering the nucleolar stress response. Nat Commun 15, 8404 (2024). https://doi.org/10.1038/s41467-024-52659-x
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
