DNA double helix
Knock-in iPS Cells

CRISPR-edited Knock-in iPS Cells

Accelerate disease model development, drug discovery, and regenerative medicine with the high editing efficiency and precise genomic integration of EditCo’s edited iPS Cells.

Service OverviewContact Us
Overview

Unlock advanced CRISPR knock-ins for neuroscience and regenerative medicine research.

Quality

Maintain iPSC quality, pluripotency, and cell integrity with EditCo’s editing process.

Precision

Focus on the phenotype of your desired edit and minimize off-target effects with transient transfection.

Unlocked Capacity

Experience faster lead times with EditCo’s high-throughput automated platform.

Human iPSC colony under fluorescence

Achieve Precise CRISPR Knock-Ins While Preserving iPSC Quality

Elevate your neuroscience, cardiovascular, or regenerative medicine research with EditCo’s precise CRISPR knock-in edits in iPSCs. Let EditCo handle the complex editing process so you can focus on assay development, differentiation, and other downstream applications. Our robust, automated platform ensures high editing efficiencies while maintaining cell quality and pluripotency, providing you with reliable and artifact-free CRISPR-edited iPSCs. EditCo offers a range of knock-ins, including single nucleotide variants (SNVs), tags, and <100 bp insertions, available in both homozygous and heterozygous states, and in clone or pool formats. Empower your research and accelerate your discoveries with EditCo’s advanced CRISPR technology.

EditCo’s Automated iPS Cell Line Editing Process

EditCo cell pools and clones editing timeline
  • Isogenic Neuronal Lines: Developing a neuronal isogenic line reverting a disease SNV genotype back to wild type or vice versa is easy with our quality CRISPR edited iPSCs differentiated into your neuronal cell type(s).
  • Drug Screening: Enable drug screening in disease-relevant cell types with necessary gene-corrected control lines to increase the rate of success of an identified target compound.
  • Endogenous Tagging of Proteins: Efficiently tag your target protein to study subcellular localization under endogenous regulatory elements.
  • Disease Modeling: Study your mutation of interest in a disease-relevant cell type through edited iPSCs that maintain quality, enabling differentiation.
Single-guide RNA Knock-in Cell Pools

Single-guide RNA Knock-in Cell Pools

High Efficiency CRISPR iPSC Knock-ins with DIY Clonal Isolation

EditCo’s Knock-in iPS Cell Pools provide a heterogeneous mix of CRISPR-edited and unedited cells, giving you high editing efficiency without clonal isolation. Benefit from EditCo’s optimized CRISPR platform, achieving precise edits while allowing you the flexibility to perform clonal isolation yourself. For those seeking a fully automated process, explore our Knock-in iPS Cell Clones for guaranteed homogeneity and convenience.

Features

Cell Source
  • EditCo supplied (standard)
  • Customer supplied
Available Edits
  • SNV, Tag, or Insertion
  • A heterogeneous population of edited and unedited cells
CRISPR Design
  • Synthetic modified sgRNA (standard)
  • Donor ssODN (standard)
Add-ons
  • QC: Pluripotency testing
  • QC: Western blot
  • QC: Whole genome sequencing

Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided

Deliverables

  • Regular updates on your order's progress
  • Edited cell pools (2 vials with 5 million cells/vial)
  • Mock-transfected cell pools (2 vials with 5 million cells/vial)
  • Sequence of synthetic sgRNA used
  • Primer sequences used for NGS sequencing
  • NGS sequencing analysis report for each edited pool after expansion.
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC

Robust Editing Across Different iPSC Lines

EditCo’s robust, automated editing platform results in high knock-in efficiency across a range of workhorse iPSC lines and patient derived iPS cell lines, ensuring a high success rate in any iPSC line.

Figure 1. Average knock-in editing efficiency by knock-in type. Knock-in efficiencies were all above 31% and performed in a variety of EditCo-supplied and customer supplied iPSC lines. Tags and Small KIs were all less than 100 bps in length. Knock-in edits were performed using RNPs and ssODNs and editing efficiency was assessed in the pool stage before proceeding to clonal isolation. DNA in each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to quantify editing efficiency.

EditCo Delivers Pluripotent Edited Cells

Quality comes first in the development of EditCo’s Knock-in iPS Cells by using our automated workflow and transient RNPs to maintain pluripotency of your cells.

Figure 2. iPS cells were assessed for standard pluripotency markers, three days post-editing.

Single-guide RNA Knock-in Cell Clones

Single-guide RNA Knock-in Cell Clones

Move Directly Into Your Functional Assays with Confidence

Streamline your research with EditCo’s Knock-in iPS Cell Clones, a homogeneous population of iPSC cells derived from single CRISPR-edited cell. Focus on your functional assays and downstream applications while we handle the entire CRISPR editing and single-cell cloning process, ensuring you receive high-quality, reliable clones to accelerate your scientific breakthroughs.

Features

Cell Source
  • EditCo supplied (standard)
  • Customer supplied
Genetic Modifications
  • SNV, Tag, or Insertion
  • Homozygous or Heterozygous Edits
CRISPR Design
  • Synthetic modified sgRNA (standard)
  • Donor ssODN (standard)
Add-Ons
  • Additional clones
  • QC: Pluripotency testing
  • QC: Karyotype testing
  • QC: Western blot
  • QC: Whole genome sequencing

Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided

Deliverables

  • Regular milestone updates on your order's progress
  • 2 independent clones with the required knock-in (2 vials of each clone with 500,000 cells/vial)
  • Mock-transfected cell pools (2 vials with 500,000 cells/vial)
  • Sequence of synthetic sgRNA and HDR template used
  • Primer sequences used for NGS sequencing
  • NGS sequencing analysis report for each edited pool after expansion.
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC

Precise Single Nucleotide Variants (SNVs)

Figure 3. 100% SNV editing in iPS cell clones. A CRISPR-edited homozygous clone containing a single nucleotide change from cytosine (C, bottom) to thymine (T, top). The SNV change is enclosed by an orange box across both traces. The target sequence (underlined in black) and the PAM site (underlined with a red dashed line) are indicated in the control trace. Human iPS cells were electroporated with sgRNA and Cas9 (as RNPs) along with a ssODN containing the single nucleotide change. To produce clonal populations, single cells were isolated using limiting dilution and expanded.

Cell Lines

Use Our iPS Cell Lines or Onboard Your Own

EditCo-supplied cell lines available for all engineered iPS cell orders at no additional cost

EditCo IDCell LineSource TissueDonor SexDonor AgeDonor EthnicityReprogramming Method
PGP1PGP1-SV1Skin — human fibroblastsMale55CaucasianSendai virus
802-30FRPChiPS8023G1Blood — human endothelial progenitor cellsFemale30HispanicmRNA
771-32MRPChiPS7713GBlood — human endothelial progenitor cellsMale32CaucasianmRNA
BX30-50MBX-30PBMCMale50AsianBlood episomal
BX32-29FBX-32PBMCFemale29AsianBlood episomal

* Parental vials available for evaluation prior to booking an edit

** Fully differentiated neurons and glia may be available from one or more of the above lines. Please inquire with BrainXell (www.brainxell.com).

Scientific Publications Featuring EditCo Knock-in iPS Cells

Resources

BrochureEditCo Capabilities BrochureDiscover how EditCo is redefining genome engineering with high-efficiency CRISPR knockouts, knock-ins, and functional tags, built for scalable, reproducible, biologically relevant results.Download Now
Application NoteCRISPR-Engineered Cells Enable Variant Disease ModelingUnderstand how all researchers can leverage CRISPR-engineered cells to model multiple genetic variants and clones at scale.Learn More
articleA New Research Toolbox at the Forefront of Genomic EngineeringThis GEN article describes how EditCo’s novel CRISPR platform is changing cell and gene therapy research, including high-throughput iPSC editing.Learn More
FlyerCRISPR-Edited iPS Cells, GuaranteedCRISPR-Edited iPS Cells, GuaranteedWe offer knockouts, single nucleotide variants, and tag insertions in control or patient-derived iPS cell lines—available in homozygous or heterozygous clone or pool formats.Learn More
EditCo’s Automated CRISPR Platform

Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.

Leveraging a sophisticated infrastructure with integration between bioinformatics, software, and automated platformsLearn More
Automating biology and synergistic disciplines for streamlined cell editing and cell culture workflowsLearn More
Delivering experiment-ready edited cells for your next discoveries through a robust and cohesive ecosystemLearn More
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