Knockout Immortalized Cell Lines
CRBN Knockout in HEK293 Cell Line (Human)
Fast, Reliable CRISPR Knockout Pools and Clones. Consistent knockouts you can trust leveraging our XDel multi-guide designs with 98% average editing efficiency. NGS-verified with QC report, assay ready cells shipped in as fast as 2 weeks. *Please contact your EditCo representative for academic pricing.
CRBN
ENSG00000113851This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
- Guides designed
- 3
- Cuts canonical
- Yes
- Coding seq. cut
- 76%–86%
- CCDS cut
- 2 of 2
- Earliest exon cut
- Exon 3
- Off-target genes
- None
Transformed cell line · Female · Fetus · kidney
No exact DepMap match for this line, so per-gene dependency/expression isn't available (only ~⅔ of lines are profiled).
Popular targets by cell line
Data
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.