Custom Tagged Knock-In Immortalized & iPS Cells
Custom HiBiT-tagged knock-in cell lines for scalable, real-time endogenous protein quantification, visualization, and degradation studies.
Custom Design. CRISPR Precision. Seamless Delivery.
HiBiT is an 11–amino acid tag that enables real-time, quantitative readout of endogenous protein expression without disrupting protein function or localization. When paired with Promega’s LgBiT and Nano-Glo® detection system, EditCo’s robust, HiBiT-tagged CRISPR knock-ins transform your cells into powerful biosensors — perfect for drug discovery, cell-based assays, and protein quantification.
Our team of experts will design a precise knock-in strategy based on your gene of interest, cell line, and insertion location preference (C-terminal, N-terminal). Using RNP electroporation and synthetic guides, we maximize editing efficiency without introducing exogenous DNA elements.
Cells available both clonally or as highly efficient pools with >70% average knock-in efficiency
Protein Localization Studies: Track subcellular proteins in real-time and understand into how proteins move within cell compartments. Get insights into how location correlates with protein function.
Protein-Protein Interaction Mapping: Tag your target protein to explore how proteins form complexes with other molecules, contributing to signal transduction, regulation, and cellular processes.
Cell Line Generation: Create tagged cell lines for high-throughput drug screening or drug target validation, observing the effects of drugs on the tagged endogenous proteins in real-time.
Protein quantification: Track expression or degradation in response to various stimuli, disease conditions, or genetic mutations to understand underlying pathways and mechanisms.
Using CRISPR Knock-ins to Monitor Protein Abundance, Internalization, and Regulation
Determining Regulated Protein Abundance
Cellular protein levels shift in response to environmental signals or therapeutic intervention — and measuring those changes is essential for understanding biology and drug response. The rise of targeted protein degradation has unlocked a new modality for drug discovery, especially for previously “undruggable” targets.
By using CRISPR/Cas9 to insert a bioluminescent tag like HiBiT at the endogenous gene locus, researchers can quantify protein abundance with precision — no antibodies or overexpression required. These assays are highly sensitive, scalable, and compatible with both endpoint and live-cell formats, enabling real-time insight into native protein dynamics.
Internalization of Cell Surface Receptors
Many cell surface receptors are internalized upon ligand binding as part of their natural signaling cycle. By inserting a HiBiT tag at the extracellular terminus of the receptor using CRISPR/Cas9, you can monitor internalization and recycling in real time — without relying on antibodies or overexpression systems.
Monitoring Downstream Target Regulation
Upstream signaling events often influence the expression of downstream genes. With CRISPR/Cas9, you can insert a HiBiT tag at the endogenous locus of a downstream target and directly measure changes in protein expression. These live-cell assays enable real-time tracking of how pathway activity translates into functional protein output — all without overexpression or complex antibody workflows.
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
