Arrayed CRISPR gRNA Libraries
Arrayed, ready-to-transfect XDel gRNA libraries for loss-of-function screening — no NGS deconvolution, with edits quantified by standard PCR or Sanger sequencing.
Accelerate target discovery with XDel Knockout Technology for more effective CRISPR screening
High Knockout Efficiency
Achieve superior on-target editing rates and consistent functional knockout performance.
Trusted Quality
Ensure confidence in every screen with our unique XDel technology and robust quality control.
Reliable Screening, Simplified
Simply quantify your knockouts with precision, while our fast turnaround times help you screen faster with ease.
Discover the New Way to Screen with XDel Technology
Other loss-of-function screening libraries require labor-intensive preparation, complex data analysis, and suffer from poor editing efficiency. Low-quality reagents can also be cytotoxic to sensitive cell types like primary cells. EditCo’s Arrayed CRISPR Synthetic Guide RNA Libraries are different.
Our XDel technology was designed to address the inefficiencies of traditional CRISPR knockout strategies. A set of predesigned guide RNAs (gRNAs) target a single early exon of a gene and work cooperatively to increase editing efficiency by creating targeted fragment deletions. Unlike standard methods that introduce random indels, our approach ensures coordinated deletions for more reliable gene disruption and complete functional knockouts.
Our libraries are ready-to-transfect, either complexed into RNPs or directly into a Cas9-expressing cell line, avoiding long preparation protocols. The arrayed format simplifies screening by removing the need for NGS deconvolution, enabling binary and multiparametric functional assays.
Additionally, the XDel design allows the knockout gene fragments to be rapidly quantified using standard PCR amplification for seamless integration into your CRISPR workflow. Analyze your edits in seconds with Sanger sequencing using our ICE tool.
Comprehensive Gene Coverage for Whole Human
Featuring our XDel Technology, we offer Whole Human Genome libraries designed for high editing efficiency and reduced false negatives. Our libraries are delivered ready-to-transfect, minimizing laborious preparation across more than 20,000 gene targets.
Our Whole Human Genome libraries enable you to get the most out of your target identification screen. If you’re more interested in studying a specific gene set or pathway, check out our User-defined or Pathway Libraries.
Complete your CRISPR screening experiment with SpCas9 nuclease and Tris-EDTA Buffer or Nuclease-free Water to rehydrate your XDel synthetic guides.
What We Provide
Features
| Species | Human |
|---|---|
| Sizes | Human - 150 pmol to 10 nmol |
| Plate Format | Human 96-well plate: 96-well Polypropylene; Human 384-well plate: 384-well Polypropylene, 384-well Echo-Validated, 384-well Echo-LDV |
Deliverables
- SpCas9 XDel guides plated library (up to 3 guides per well), dry
- Synthetic guides are modified (2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases)
- Modifications help resist degradation and prevent triggering intracellular immune responses
- Library plate layouts (.csv)
- QC document (pdf) and guides and primer sequences for PCR and Sanger sequencing .csv file
- User selected add-on controls
Quotes and ordering
If you need a quote to place your order, request a quote by clicking below.
Transform Your Target Discovery With Our Pathway Libraries
We offer 30+ Pathway Libraries including druggable, GPCRs, kinases, and immuno-oncology targets. These libraries are well-suited for target identification studies, providing a comprehensive gene set to begin your discovery process. Select Pathway libraries are shipped in as quickly as one week so you can begin your screen even sooner. Our Pathway Libraries feature our XDel design to create predictable knockouts with high-efficiency and reduce false negatives. See the table below for a full list of available Pathway Libraries - Contact us to order. If you are interested in more widespread coverage, check out our Whole Genome Libraries. If you are interested in a specific set of targets, check out our User-defined Libraries.
Contact our sales team for more information about our libraries.
Complete your CRISPR screening experiment with SpCas9 nuclease and Tris-EDTA Buffer or Nuclease-free Water to rehydrate your XDel synthetic guides.
Available Gene Pathways
| Pathway Libraries List | Number of Genes in Library |
|---|---|
| Apoptosis Pathway Library | 1997 |
| Autophagy Library | 460 |
| B-Cell Activation Pathway Library | 242 |
| Cell Adhesion Genes Library | 1479 |
| Cell Cycle Regulators Library | 1251 |
| Cell Surface Proteins Library | 2738 |
| Complete Druggable Library | 8478 |
| Kinase Library | 1035 |
| Cytokines and Chemokines Library | 310 |
| Cytoskeleton Genes Library | 1745 |
| Deubiquitinating Enzymes Library | 117 |
| DNA Repair Pathway Library | 530 |
| Epigenetic Regulators Library | 826 |
| Essential Genes Library | 2553 |
| Extracellular Matrix Genes Library | 363 |
| G-Protein Coupled Receptors Library | 1046 |
| Helicases Library | 154 |
| Immunology/Immuno-Oncology Library | 3098 |
| Ion Channels Library | 685 |
| JAK-STAT Pathway Library | 194 |
| Metabolic Activity Library | 11432 |
| Nuclear Hormone Receptors Library | 192 |
| p53 Pathway Library | 319 |
| Pattern Recognition Receptors and Signaling Pathways Library | 303 |
| Phosphatases Library | 344 |
| SARS-CoV-2 Druggable Interactome Library | 63 |
| SARS-CoV-2 Interactome Library | 324 |
| Secreted Proteins Library | 5292 |
| Serine Proteases Library | 263 |
| T-Cell Activation Pathway Library | 524 |
| Transcription Factors Library | 2358 |
| Tumor Suppressors Library | 1055 |
| Tyrosine Kinases Library | 152 |
| Ubiquitin Ligases Activity (E1, E2, E3) Library | 1159 |
| Ubiquitin Protein Ligases Library | 336 |
What We Provide
Features
| Species | Human |
|---|---|
| Sizes | Human - 150 pmol to 10 nmol |
| Plate Format | Human 96-well plate: 96-well Polypropylene; Human 384-well plate: 384-well Polypropylene, 384-well Echo-Validated, 384-well Echo-LDV |
Deliverables
- SpCas9 XDel guides plated library (up to 3 guides per well), dry
- Synthetic guides are modified (2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases)
- Modifications help resist degradation and prevent triggering intracellular immune responses
- Library plate layouts (.csv)
- Guides and primer sequences for PCR and Sanger sequencing .csv file
Quotes and Ordering
If you need a quote to place your order, request a quote by clicking below.
Confidently Knock Out Your Target Set of Genes
As part of our XDel arrayed knockout libraries, we also offer User-defined Libraries so you can choose your specific gene set from human and mouse genomes, well-suited for target validation studies. Human User-defined Libraries are shipped in as quickly as one week. Our User-defined Libraries feature our XDel technology to create predictable knockouts with high-efficiency and reduce false negatives. All libraries arrive ready-to-transfect, eliminating laborious and time-intensive steps that alternative libraries require, and can be used to screen in any human or mouse cell type, including primary cells.
Complete your CRISPR screening experiment with SpCas9 nuclease and Tris-EDTA Buffer or Nuclease-free Water to rehydrate your XDel synthetic guides.
What We Provide
Features
| Species | Human; Mouse |
|---|---|
| Sizes | Human - 150 pmol to 10 nmol; Mouse - 1500 pmol |
| Plate Format | Human 96-well plate: Nunc Deepwell; Human 384-well plate: Nunc, ECHO PP, or ECHO LDV; Mouse 96-well plate: Nunc Deepwell |
Deliverables
- SpCas9 XDel guides plated library (up to 3 guides per well), dry
- Synthetic guides are modified (2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases)
- Modifications help resist degradation and prevent triggering intracellular immune responses
- Library plate layouts (.csv)
- QC document (pdf) and guides and primer sequences for PCR and Sanger sequencing .csv file
- User selected add-on controls
Quotes and ordering
If you need a quote to place your order, request a quote by clicking below.
Optimal Knockout Efficiency for High-throughput
How does the XDel strategy work?
Traditional CRISPR knockout methods often depend on a single guide RNA that, in tandem with SpCas9, generates assorted insertions or deletions (indels) at the target cut site. This approach can be unpredictable, leading to variable edits and incomplete knockouts.
EditCo’s smart informatics generates a multi-guide design which is composed of up to 3 sgRNAs targeting a single gene of interest. The guides are spatially coordinated and work cooperative to induce a guided repair that results in fragment deletion in an early exon, making it the most reliable knockout strategy compared to other pooled strategies.
XDel Technology Consistently Induces Fragment Deletions
Figure 3. XDel multiple gRNA creates fragment deletions. Individual vs. XDel gRNA editing was compared for 2 gene targets (TNF, TLR4) in dendritic cells (transfected via nucleofection). Editing efficiency was analyzed by sequencing the targeted loci on a MiSeq and sequencing outcomes were categorized based on editing type (no indel, large deletion ≥50bp, small deletion <50bp, insertion). Data generated by Dr. Marco Jost and Dr. Jonathan Weissman, University of California, San Francisco, and Dr. Amy Jacobson and Dr. Michael Fischbach, Stanford University.
XDel Achieves Higher and More Consistent On-Target Editing in Immortalized, iPSC, and Primary Cells Compared to Single-Guide
Figure 4. XDel multiple gRNA on-target editing efficiency is significantly higher and more consistent compared to single-guide RNA methods. On-target editing efficiency of multi-guide (pink bars) vs their 3 respective single-guide RNAs (blue bars) across 7 endogenous target loci (with known high off-target editing sites) transfected in two immortalized (IMM) and two iPSC cell lines indicates that XDel technology delivers significantly higher and more consistent on-target editing efficiency compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.
XDel technology maximizes on-target gene editing while minimizing off-target effects compared to single-guide editing
Figure 5. XDel multiple guide RNA off-target editing efficiency is significantly lower compared to single guide RNA methods when targeting endogenous sites known for their high off-target levels. Off-target editing efficiency of XDel cells (pink bars) at all 3 of their respective single-guide off-target sites vs their 3 respective single-guide RNAs (blue bars) across 63 off-target loci (3 off-target loci per 7 on-target sites tested) transfected in two immortalized (IMM) and two iPSC cell lines indicates that XDel cells deliver significantly lower off-target editing efficiency compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.
Figure 6. XDel multiple guide RNA editing efficiency remains high as gRNA concentration is decreased compared to single guide RNA methods. Average on-target (pink bars, multi-guide; blue bars, single-guide) editing efficiency in transfected HEK293 cells with multi-guide (left) vs single-guide (right) and SpCas9 at increasing RNP concentrations (0.25X, 1X, 4X) at 7 endogenous sites vs off-target editing efficiency (stacked grey bars) at 63 off-target sites indicates that XDel cells maintain high on-target editing efficiency with minimal off-target effects compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.
Edited Cells with XDel Technology Demonstrate Sustained Gene Knockout Across Multiple Cell Passages
Figure 7. High editing efficiency is sustained through multiple cell passages. Using EditCo’s multi-guide designs, up to 3 modified gRNA per target (across 6 genes) were conjugated with SpCas9, and RNPs were transfected into U2OS cells. Editing efficiencies were measured at different time points post-transfection and up to 4 passages. The median editing efficiency is >85 percent for each of the targets.
Cell Viability Is Maintained Across Passages with XDel
Figure 8. Cell viability is maintained through multiple cell passages in U2OS cells. Viability was taken using Celigo. Please note that CDK9 is a common essential gene, hence the lower viability and cell number observed along the passages.
XDel Technology Result in Sustained Protein Depletion
Figure 9. Edited cell pools utilizing the multi-guide technology shows persistent protein depletion. This enables direct and reliable use of these cell pools for functional assays. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.
Achieve Robust Genotype and Phenotype Screening Results with XDel
Figure 10. Across 77 genes, arrayed XDel library resulted in an average Knockout Score of 75%. Using our multi-guide algorithm, up to 3 modified gRNA per target (across 86 genes) were transfected into U2OS-Cas9 expressing cell line using nucleofection. This resulted in an average Knockout Score of 75% for 77 of the genes. Nine of the 86 genes failed due to sequencing errors.
EditCo Libraries Enables High Editing Efficiencies in a Large-scale Primary Cell Screen
Figure 11. 97% median editing efficiency using across 280 loci in primary dendritic cells. XDel gRNA was transfected into primary dendritic cells using nucleofection. Editing efficiency was assessed via NGS across 280 loci. Data courtesy of Weissman Lab, UCSF.
Resources
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
Reach out to our experts to find out what EditCo can do for you
Data
Achieve Robust Genotype and Phenotype Screening Results with XDel Knockout TechnologyFigure 1. A) Across 77 genes, the arrayed XDel library achieved an average Knockout Score of 75%. A total of 86 genes were transfected into U2OS-Cas9 cells via nucleofection. Nine of the 86 genes failed due to sequencing errors. B) Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.