Knockout Immortalized Cell Lines
TP53 Knockout in HEK293 Cell Line (Human)
Fast, Reliable CRISPR Knockout Pools and Clones. Consistent knockouts you can trust leveraging our XDel multi-guide designs with 98% average editing efficiency. NGS-verified with QC report, assay ready cells shipped in as fast as 2 weeks. *Please contact your EditCo representative for academic pricing.
TP53
ENSG00000141510This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
- Guides designed
- 3
- Cuts canonical
- Yes
- Coding seq. cut
- 54%–66%
- CCDS cut
- 26 of 26
- Earliest exon cut
- Exon 5
- Off-target genes
- None
Transformed cell line · Female · Fetus · kidney
No exact DepMap match for this line, so per-gene dependency/expression isn't available (only ~⅔ of lines are profiled).
Popular targets by cell line
Data
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.