CRISPR Reagents

Gene Knockout Kits

Knockout any human or mouse protein-coding gene with XDel technology.

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Overview

Simple and reliable CRISPR knockout kits using XDel technology for any human or mouse protein-coding gene

Faster Data

Get your Gene Knockout kit in as fast as 2 days for human genes, with typical delivery in 5 days. Quickly verify knockout efficiency with standard PCR amplification.

High Efficiency

Achieve superior on-target editing rates and consistent knockout performance, guaranteed.

Simplified Online Ordering

Easily order online through our ordering portal. Complete your experiment with controls, SpCas9 nuclease and Transfection Optimization kit.

Researcher preparing CRISPR reagents

Human and Mouse Knockouts Have Never Been Easier with XDel Technology.

EditCo’s Gene Knockout Kit was designed to increase the efficacy of CRISPR-mediated knockouts. To address the inefficiencies of traditional CRISPR knockout strategies, we developed a solution: a set of predesigned guide RNAs (gRNAs) targeting a single early exon of a gene that work cooperatively to increase editing efficiency by creating targeted fragment deletions.

Unlike the standard approach, which relies on a single gRNA to introduce random indels at one site—or multiple gRNAs that randomly target different regions—our method is specifically designed to create coordinated deletions that reliably disrupt the gene. This approach improves the likelihood of generating a complete functional knockout, reducing the unpredictability and inefficiency associated with traditional strategies.

In addition, the resulting knockout gene fragment can then be rapidly and accurately quantified using a standard PCR amplification to ensure seamless integration into your CRISPR workflow. Analyze your CRISPR edits in seconds using Sanger sequencing with our ICE tool.

XDel design with up to 3 modified sgRNAs targeting a single gene
Figure 1. XDel design includes up to 3 modified sgRNAs (grey bars) that target a single gene of interest. When co-transfected, the sgRNAs create concurrent double-stranded breaks at the targeted genomic locus and consequently induce one or more 21+ bp fragment deletions.
Deliverables

Gene Knockout Kits for any human or mouse genes

Our Gene Knockout Kit is guaranteed for human and mouse (excluding genes deemed Common Essential, per the DepMap Database).

The multiple gRNAs are chemically modified to resist degradation and prevent triggering intracellular immune responses.

Complete your CRISPR experiment with the Transfection Optimization Kit, add-on controls and SpCas9 nuclease.

What We Provide

Features

SpeciesHuman, Mouse
Sizes1.5, 3, 5, 10 nmol
FormatIndividual tubes

Deliverables

  • Target-specific XDel guides (up to 3 guides per target), Tube, dry
  • Synthetic guides are modified (2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases)
  • Modifications help resist degradation and prevent triggering intracellular immune responses
  • Nuclease Free Water 1.5 ml (tube) (1 for every 5 kits)
  • TE Buffer 1.5 ml (tube) (1 for every 5 kits)
  • QC document (pdf) and guides and primer sequences for PCR and Sanger sequencing .csv file
Data

XDel Technology Consistently Induces Fragment Deletions

Figure 2. XDel multiple gRNA creates fragment deletions. Individual vs. XDel gRNA editing was compared for 2 gene targets (TNF, TLR4) in dendritic cells (transfected via nucleofection). Editing efficiency was analyzed by sequencing the targeted loci on a MiSeq and sequencing outcomes were categorized based on editing type (no indel, large deletion ≥50bp, small deletion <50bp, insertion). Data generated by Dr. Marco Jost and Dr. Jonathan Weissman, University of California, San Francisco, and Dr. Amy Jacobson and Dr. Michael Fischbach, Stanford University.

XDel Achieves Higher and More Consistent On-Target Editing in Immortalized, iPSC, and Primary Cells Compared to Single-Guide

Figure 3. XDel multiple gRNA on-target editing efficiency is significantly higher and more consistent compared to single-guide RNA methods. On-target editing efficiency of multi-guide (pink bars) vs their 3 respective single-guide RNAs (blue bars) across 7 endogenous target loci (with known high off-target editing sites) transfected in two immortalized (IMM) and two iPSC cell lines indicates that XDel technology delivers significantly higher and more consistent on-target editing efficiency compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.

XDel technology maximizes on-target gene editing while minimizing off-target effects compared to single-guide editing

Figure 4. XDel multiple guide RNA off-target editing efficiency is significantly lower compared to single guide RNA methods when targeting endogenous sites known for their high off-target levels. Off-target editing efficiency of XDel cells (pink bars) at all 3 of their respective single-guide off-target sites vs their 3 respective single-guide RNAs (blue bars) across 63 off-target loci (3 off-target loci per 7 on-target sites tested) transfected in two immortalized (IMM) and two iPSC cell lines indicates that XDel cells deliver significantly lower off-target editing efficiency compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.

Figure 5. XDel multiple guide RNA editing efficiency remains high as gRNA concentration is decreased compared to single guide RNA methods. Average on-target (pink bars, multi-guide; blue bars, single-guide) editing efficiency in transfected HEK293 cells with multi-guide (left) vs single-guide (right) and SpCas9 at increasing RNP concentrations (0.25X, 1X, 4X) at 7 endogenous sites vs off-target editing efficiency (stacked grey bars) at 63 off-target sites indicates that XDel cells maintain high on-target editing efficiency with minimal off-target effects compared to single-guide. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis.

Edited Cells with XDel Technology Demonstrate Sustained Gene Knockout Across Multiple Cell Passages

Figure 6. High editing efficiency is sustained through multiple cell passages. Using EditCo’s multi-guide designs, up to 3 modified gRNA per target (across 6 genes) were conjugated with SpCas9, and RNPs were transfected into U2OS cells. Editing efficiencies were measured at different time points post-transfection and up to 4 passages. The median editing efficiency is >85 percent for each of the targets.

Cell Viability Is Maintained Across Passages with XDel

Figure 7. Cell viability is maintained through multiple cell passages in U2OS cells. Viability was taken using Celigo. Please note that CDK9 is a common essential gene, hence the lower viability and cell number observed along the passages.

XDel Technology Result in Sustained Protein Depletion

Figure 8. Edited cell pools utilizing the multi-guide technology shows persistent protein depletion. This enables direct and reliable use of these cell pools for functional assays. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.

Scientific Publications Featuring EditCo Gene Knockout Kits

Resources

BrochureEditCo Capabilities BrochureDownload our Capabilities Brochure to see how EditCo delivers high-precision CRISPR knockouts, knock-ins, and functional tags at scale, engineered for speed, reproducibility, and real biological insight.Download Now
The EDITorialNot All CRISPR Kits Are Created Equal: How to Choose the Right Knockout KitIn our latest Editorial, we explore key factors, like efficiency, ease of use, and cell type compatibility, to help you choose the CRISPR knockout kit that best fits your research.Read More
Application NoteOptimized CRISPR/Cas9 Gene Knockout: XDel technology maximizes on-target editing and minimizes off-target effectsLearn how EditCo Bio’s XDel CRISPR technology enables efficient, high-fidelity gene knockouts with minimal off-target effects, validated across multiple cell types. Download the application note to explore the data.Download Now
FlyerGene Knockout Kit: Elevate Your Guide Design StrategyEditCo’s Gene Knockout Kit takes the trial and error out of CRISPR knockouts by using a novel strategy of guide RNA design to knock out any human or mouse protein-coding gene.Download Now
Application NoteXDel Technology Improves CRISPR Knockout Efficiency with Single Exon Fragment DeletionsLearn how intelligently designed multiple gRNAs improve CRISPR knockout efficiency with single exon fragment deletions.Learn More
Quick Start GuideGene Knockout Kit Quick Start GuideThis quick-start guide can be used for EditCo’s Gene Knockout Kit. In this guide, we include important information on how to store, quantify, and transfect your gRNA kit and analyze your knockout kit experiments.Learn More
Quick Start GuideTransfection Optimization Kit (Multi-guide)EditCo’s Transfection Optimization Kit (Multi-guide) is a positive control kit that is meant to be used in conjunction with EditCo’s Gene Knockout Kit.Download Now
E-bookCell Engineering 101 eBookDiscover methods and insights into skillfully generating knockout or knock-in CRISPR edits in a wide-range of cell lines.Learn More
EditCo’s Automated CRISPR Platform

Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.

Leveraging a sophisticated infrastructure with integration between bioinformatics, software, and automated platformsLearn More
Automating biology and synergistic disciplines for streamlined cell editing and cell culture workflowsLearn More
Delivering experiment-ready edited cells for your next discoveries through a robust and cohesive ecosystemLearn More
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