Application Note

Intelligently Designed XDel Technology Improves CRISPR Knockout Efficiency with Single Exon Fragment Deletions

Learn how EditCo’s XDel Technology is game-changing CRISPR

How do you create a novel CRISPR design method that dramatically increases knockout efficiency?

The EditCo team has used our learnings from over 300,000 CRISPR edits to refine our XDel Technology to improve your CRISPR editing.

Our application note delves into the power of CRISPR-Cas9 in gene knockouts, highlighting EditCo’s innovative XDel approach. With higher knockout efficiency and complete protein-level knockouts, this method is set to accelerate your functional genomics and disease modeling projects.

Key findings

EditCo’s multi-guide sgRNA uses 3 sgRNAs targeted to a single exon of the gene of interest.
Generating 21+ bp deletions in targeted regions results in a higher knockout efficiency than any individual guide alone.
Complete protein-level knockouts were consistently observed in non-clonal cell populations after editing by multi-gRNA.

Ready to elevate your research? Download our application note today and explore how EditCo’s XDel gene editing can transform your genetic studies!