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Versatile and reliable edited iPS Cells for groundbreaking discoveries

Accelerate disease model development, drug discovery, and regenerative medicine with the high editing efficiency and precise genomic integration of EditCo's edited iPS Cells.

Let's talk about how our iPS Cells can help your research.
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Overview

Accurate, efficient models for advanced disease research.

  • Quality: iPSC quality, pluripotency, and cell integrity are maintained through EditCo’s editing process.
  • Unparalleled Editing Efficiency: Our automated, optimized platform and superior quality reagents result in high-efficiency editing.
  • High Success Rates: EditCo’s robust CRISPR editing process consistently delivers functional gene knockouts.
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Experience Unmatched CRISPR Knockout Efficiency in iPSCs

Discover the one-stop solution for reliable and precise knockouts in induced pluripotent stem cells (iPSCs) with EditCo's Engineered Knockout iPS Cells. Our advanced CRISPR editing technology ensures consistently high knockout efficiency (>95%), achieving near-complete functional knockouts even before clonal selection. Utilizing high-quality synthetic non-integrating reagents, our proprietary automated process maintains cell viability and pluripotency, ensuring your cells are artifact-free and your experimental integrity remains intact. Choose EditCo’s Engineered Knockout iPS Cells, available as 100% knockout guaranteed clones or as efficient, non-sorted pools, to elevate your gene function and disease linkage studies. Unlock unprecedented potential and accelerate your research with EditCo.

EditCo’s Automated iPS Cell Line Editing Process

Cell Pools Clones Timeline

 

Gene Function
Gene Function Studies: Functionally disrupt your target gene to confirm if it is necessary for a specific phenotype or cellular function.
Gene-Disease
Gene-Disease Linkage: Investigate the genetic factors involved in the pathogenesis of neurodegenerative diseases with edited iPSCs differentiated into a neuronal cell type of interest.
Pathway Analysis
Pathway Analysis: Systemically knockout genes in your disease pathway and differentiate cells into disease-relevant cell types to assay to identify ideal drug targets.
Target Validation
Target Validation: Validate your identified targets in CRISPR-edited iPSCs to confirm gene-disease linkage in biologically relevant cell models.
Clone

Guaranteed iPSC CRISPR Knockout, Ready for Downstream Applications

EditCo’s Knockout iPS Cell Clones offer a homogeneous population of induced pluripotent stem cells with your desired protein functionally knocked out, guaranteed. These clones are the simplest way to harness the power of CRISPR in your lab, enabling you to focus on your research and downstream applications with confidence.

Features

Cell Source

  • EditCo supplied (standard)
  • Customer supplied

Available Edits

  • Indel (standard) or fragment deletion
  • Homozygous (standard) or heterozygous edits

CRISPR Design

  • Synthetic modified sgRNA (standard)
  • Multiple synthetic modified sgRNAs

Add-Ons

  • Additional clones
  • QC: Pluripotency Testing
  • QC: Karyotype Testing

Deliverables

  • Regular updates on your order's progress
  • 2 independent clones with the required knockout (2 vials of each clone with 5x10⁵ cells/vial)
  • Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
  • Sequence of synthetic sgRNA used
  • Primer sequences used for PCR and Sanger Sequencing
  • ICE Sanger sequencing analysis reports for each edited clone after expansion
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Pool

Pre-clonal iPSC CRISPR Knockout Cells: Your Unique Assay Validation Tool

EditCo’s Knockout iPS Cell Pools offer a heterogeneous population of induced pluripotent stem cells. Each pool contains iPSCs edited to knock out your gene of interest through CRISPR transfection and a population of unedited cells. Delivered prior to clonal isolation, these pools combine the benefits of EditCo’s optimized CRISPR editing process with high knockout efficiency. For a homogeneous cell population ready for immediate use, explore our Knockout iPS Cell Clones with automated clonal isolation.

Features

Cell Source

  • EditCo supplied (standard)
  • Customer supplied

Available Edits

  • Indel (standard) or fragment deletion
  • A heterogeneous population of edited and unedited cells

CRISPR Design

  • Synthetic modified sgRNA (standard)
  • Multiple synthetic modified sgRNAs

Add-Ons 

  • QC: Pluripotency testing

Deliverables

  • Regular updates on your order's progress
  • Edited cell pools (2 vials with 5x10⁵ cells/vial)
  • Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
  • Sequence of synthetic sgRNA used
  • Primer sequences used for PCR and Sanger Sequencing
  • ICE Sanger sequencing analysis reports for each edited clone after expansion
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Data

Unprecedented Knockout Editing Efficiency

EditCo’s Knockout iPS Cells deliver reliable protein knockout enabling your gene function studies.

iPSC Knockout

Figure 1. Modified gRNAs were transfected along with Cas9 as RNPs to knock out RELA in four individual iPS cell lines. Each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to determine editing efficiency.

 

EditCo Delivers Pluripotent Edited Cells

EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.

iPSC Pluripotency

Figure 2. iPS cells were assessed for standard pluripotency markers, three days post-editing.

 

Validate your Assays with up to 95% Protein Knockout

EditCo’s Knockout Cell Pool gives you the protein knockout you need in the cell line you want, enabling you to either assay pools directly or quickly move to isolate single cell clones.

Gene Protein Pool KO

Figure 3. EditCo’s Knockout Cell Pool achieved 95% protein knockout without clonal expansion. A cell pool with a surface protein knocked out was generated in U2OS cells with a Knockout Score of 87% (left panel, which is the percentage of indels that either introduce a frameshift mutation or are larger than 21 nucleotides, analyzed by ICE). Levels of this protein were analyzed by flow cytometry (blue) and were reduced by 95% relative to wild type (green, right panel). Negative control samples were stained with an isotype control antibody (purple). Data provided by Eurofins-DiscoverX.

Resources

Article
A New Research Toolbox at the Forefront of Genomic Engineering
This GEN article describes how EditCo’s novel CRISPR platform is changing cell and gene therapy research, including high-throughput iPSC editing.
Learn More
Application Note
CRISPR-Engineered Cells Enable Variant Disease Modeling
Understand how all researchers can leverage CRISPR-engineered cells to model multiple genetic variants and clones at scale.
Learn More
Flyer
CRISPR-Edited iPS Cells, Guaranteed
We offer knockouts, single nucleotide variants, and tag insertions in control or patient-derived iPS cell lines—available in homozygous or heterozygous clone or pool formats.
Learn More
Overview

Unlock advanced CRISPR knock-ins for neuroscience and regenerative medicine research.

  • Quality: Maintain iPSC quality, pluripotency, and cell integrity with EditCo’s editing process.
  • Precision: Focus on the phenotype of your desired edit and minimize off-target effects with transient transfection.
  • Unlocked Capacity: Experience faster lead times with EditCo’s high-throughput automated platform.
iPSC3

Achieve Precise CRISPR Knock-Ins While Preserving iPSC Quality

Elevate your neuroscience, cardiovascular, or regenerative medicine research with EditCo's precise CRISPR knock-in edits in iPSCs. Let EditCo handle the complex editing process so you can focus on assay development, differentiation, and other downstream applications. Our robust, automated platform ensures high editing efficiencies while maintaining cell quality and pluripotency, providing you with reliable and artifact-free CRISPR-edited iPSCs.

EditCo offers a range of knock-ins, including single nucleotide variants (SNVs), tags, and <100 bp insertions, available in both homozygous and heterozygous states, and in clone or pool formats. Empower your research and accelerate your discoveries with EditCo's advanced CRISPR technology.

EditCo’s Automated iPS Cell Line Editing Process

CellPoolsClones_Timeline

 

Isogenic Neuronal Lines
Isogenic Neuronal Lines: Developing a neuronal isogenic line reverting a disease SNV genotype back to wild type or vice versa is easy with our quality CRISPR edited iPSCs differentiated into your neuronal cell type(s).
Drug Screening
Drug Screening: Enable drug screening in disease-relevant cell types with necessary gene-corrected control lines to increase the rate of success of an identified target compound.
Protein Tagging
Endogenous Tagging of Proteins: Efficiently tag your target protein to study subcellular localization under endogenous regulatory elements.
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Disease Modeling: Study your mutation of interest in a disease-relevant cell type through edited iPSCs that maintain quality, enabling differentiation.
Clone

Move Directly Into Your Functional Assays with Confidence

Streamline your research with EditCo’s Knock-in iPS Cell Clones, a homogeneous population of iPSC cells derived from single CRISPR-edited cell. Focus on your functional assays and downstream applications while we handle the entire CRISPR editing and single-cell cloning process, ensuring you receive high-quality, reliable clones to accelerate your scientific breakthroughs.

Features

Cell Source

  • EditCo supplied (standard)
  • Customer supplied

Genetic Modifications

  • SNV, Tag, or Insertion
  • Homozygous or Heterozygous Edits

CRISPR Design

  • Synthetic modified sgRNA (standard)
  • Donor ssODN (Standard)

Add-Ons

  • Additional clones
  • QC: Pluripotency Testing
  • QC: Karyotype Testing

Deliverables

  • Regular milestone updates on your order's progress
  • 2 independent clones with the required knock-in (2 vials of each clone with 5 million cells/vial)
  • Mock-transfected cell pools (2 vials with 5 million cells/vial)
  • Sequence of synthetic sgRNA and HDR template used
  • Primer sequences used for PCR and Sanger Sequencing
  • ICE Sequencing analysis reports for each edited clone after expansion
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Pool

High Efficiency CRISPR Knock-ins with DIY Clonal Isolation

EditCo’s Knock-in iPS Cell Pools provide a heterogeneous mix of CRISPR-edited and unedited cells, giving you high editing efficiency without clonal isolation. Benefit from EditCo’s optimized CRISPR platform, achieving precise edits while allowing you the flexibility to perform clonal isolation yourself. For those seeking a fully automated process, explore our Knock-in iPS Cell Clones for guaranteed homogeneity and convenience.

Features

Cell Source

  • EditCo supplied (standard)
  • Customer supplied

Available Edits

  • SNV, Tag, or Insersion
  • A heterogeneous population of edited and unedited cells

CRISPR Design

  • Synthetic modified sgRNA (standard)
  • Donor ssODN (standard)

Add-ons

  • QC: Pluripotency testing

Deliverables

  • Regular updates on your order's progress
  • Edited cell pools (2 vials with 5 million cells/vial)
  • Mock-transfected cell pools (2 vials with 5 million cells/vial)
  • Sequence of synthetic sgRNA used
  • Primer sequences used for PCR and Sanger Sequencing
  • ICE Sanger sequencing analysis reports for each edited clone after expansion
  • Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Data

Precision Knock-in Clones and Pools

Robust Editing Across Different iPSC Lines

EditCo’s robust, automated editing platform results in high knock-in efficiency across a range of workhorse iPSC lines and patient derived iPS cell lines, ensuring a high success rate in any iPSC line.

 

iPSC Knock-in

Figure 1. Average knock-in editing efficiency by knock-in type. Knock-in efficiencies were all above 31% and performed in a variety of EditCo-supplied and customer supplied iPSC lines. Tags and Small KIs were all less than 100 bps in length. Knock-in edits were performed using RNPs and ssODNs and editing efficiency was assessed in the pool stage before proceeding to clonal isolation. DNA in each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to quantify editing efficiency.

 

100% SNV Editing in iPS Cell Clones

Focus on your research by letting EditCo deliver your precise SNV knock-ins enabling creation of isogenic lines.

iPSC SNV

Figure 2. A CRISPR-edited homozygous clone containing a single nucleotide change (top) relative to the wild type control (bottom). An orange box encloses the single nucleotide change from cytosine (C) to thymine (T). The target sequence is underlined in white and the PAM site is underlined with a red dashed line in the control trace. SNV knock-ins are also available as pools.

 

EditCo Delivers Pluripotent Edited Cells

Quality comes first in the development of EditCo’s Knock-in iPS Cells by using our automated workflow and transient RNPs to maintain pluripotency of your cells.

iPSC Pluripotency

Figure 3. iPS cells were assessed for standard pluripotency markers, three days post-editing.

Resources

Application Note
CRISPR-Engineered Cells Enable Variant Disease Modeling
Understand how all researchers can leverage CRISPR-engineered cells to model multiple genetic variants and clones at scale.
Learn More
article
A New Research Toolbox at the Forefront of Genomic Engineering
This GEN article describes how EditCo’s novel CRISPR platform is changing cell and gene therapy research, including high-throughput iPSC editing.
Learn More
Flyer
CRISPR-Edited iPS Cells, Guaranteed
CRISPR-Edited iPS Cells, GuaranteedWe offer knockouts, single nucleotide variants, and tag insertions in control or patient-derived iPS cell lines—available in homozygous or heterozygous clone or pool formats.
Learn More
EditCo's Automated CRISPR Platform

Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.

CRISPR Engineered Cells Enable Variant Disease Modeling
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Leveraging a sophisticated infrastructure with integration between bioinformatics, software, and automated platforms
Learn More
Icon_TCell_Trans
Automating biology and synergistic disciplines for streamlined cell editing and cell culture workflows
Learn More
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Delivering experiment-ready edited cells for your next discoveries through a robust and cohesive ecosystem
Learn More
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Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
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Amanda Peyton, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute
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Joseph James, Ph. D.
Principal Investigator, Boston Children's Hospital
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Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
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Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
Congue fermentum eros leo commodo. Integer vitae vitae ac amet. Vitae sed lectus vel lacus adipiscing nulla facilisis mauris porttitor. Viverra elementum odio vitae id.
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Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
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