Skip to content

CRISPR Reagents: Streamline your editing with the most reliable and flexible Gene Knockout Kits and Arrayed gRNA Libraries

EditCo’s innovative approach to smart guide design allows efficient gene disruption generation, eliminating the trial and error associated with common guide design strategies. All our reagents are designed to simplify your research, ensuring more accurate and reliable results.

Streamline your CRISPR with our intuitive, flexible ordering portal.
Order Now
Overview

The most reliable CRISPR knockout strategy for any human or mouse-derived cell lines

  • Faster Data: Discover faster and eliminate the trial and error of guide screening. Get your Gene Knockout kit delivered in 5 days.
  • Efficiency: The most reliable knockout strategy. High knockout efficiency, guaranteed.
  • Simplified Online Ordering: Easily order online through our ordering portal. Complete your experiment with controls, SpCas9 nuclease and Transfection Optimization kit 
national-cancer-institute-c-wqrSCjVf4-unsplash

Human and Mouse Knockouts Have Never Been Easier.

Common CRISPR knockout strategies rely on an individual guide RNA (gRNA) that produces random indels in the gene of interest. This approach produces a single double-strand break at the targeted locus and relies on the cellular repair mechanism to generate a frameshift mutation to disrupt the gene. This strategy is inefficient due to the wide variety and unpredictability of edits that can occur and often does not generate a functional knockout.

EditCo’s Gene Knockout Kit was designed to increase the efficacy of CRISPR-mediated knockouts. Typical guide RNA strategies rely on the generation of indels, which does not always result in complete gene disruption. EditCo’s multi-guide approach consistently generates fragment deletions at the targeted loci, so you can be confident in your gene knockout. 

Multi-Guide Schematic

 

Deliverables

Gene Knockout Kits for any human or mouse genes

Our Gene Knockout Kit is guaranteed for human and mouse (excluding non-essential genes).
The multi-guide sgRNA is chemically modified to resist degradation and prevent triggering intracellular immune responses.

Complete your CRISPR experiment with the Transfection Optimization Kit, add-on controls and SpCas9 nuclease.

Features

Species
  • Human
  • Mouse
Sizes
  • 1.5, 3, 5, 10 nmol
Format
  • Individual tubes

Deliverables

  • nmol target-specific multiguide sgRNA, tube, dry 
  • sgRNAs are modified: 2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases (modifications help resist degradation and prevent triggering intracellular immune responses).
  • SpCas9 proprietary EZ Scaffold
  • Nuclease-free water 1.5 ml (tube) (1 for every 5 kits)
  • TE Buffer 1.5 ml (tube) (1 for every 5 kits)
  • QC document (pdf) and sgRNA and Primer sequences for PCR and Sanger sequencing .csv file
Data

Smart Guide Design for Guaranteed Knockouts

Multi-guide Design Consistently Induces Deletions

multi-guide-del

Figure 1. EditCo’s Gene Knockout Kit was designed to increase the efficacy of CRISPR-mediated knockouts. The multi-guide approach consists of 3 gRNAs spatially coordinated to create a fragement deletion in a single exon. Targeting a single early exon of a gene induces multiple concurrent double-strand breaks in the target sequence of a gene. This results in disruptive one or more >21 bp fragment deletions. This approach is associated with higher frequencies of knockout alleles in edited pools but reduced occurrence of off-target edits as compared to single-guide approaches.

 

GKOv2_joststackedBar-editco-4
Figure 2. Multi-guide gRNA for each target resulted in >75% large deletion outcomes. Individual vs. multi-guide gRNA editing was compared for 2 gene targets (TNF, TLR4) in dendritic cells (transfected via nucleofection). Editing efficiency was analyzed by sequencing the targeted loci on a MiSeq and sequencing outcomes were categorized based on editing type (no indel, large deletion ≥50bp, small deletion <50bp, insertion). 

 

Consistent and Reliable Knockouts

 

EB-Libraries Multi-guide Scatter

Figure 3. Better knockout efficiency was found across 32 genetic targets assessed. Guide RNAs displayed 29.2% better median knockout efficiency when introduced in a multiguide format (89.9% KO score) relative to an individual sgRNA format (69.6% KO Score).

 

Fragment Deletions Result in Sustained Protein Depletion

Fragment deletions caused by multi-guide sgRNA editing result in protein knockout after seven days post-transfection in non-clonal cell lines.

Figure 4. Editing of three genes (CDK9, CINP, COASY) in HEK293 cells (transfected via nucleofection) using the multi-guide approach resulted in high knockout efficiencies, as indicated by Knockout (KO) Scores. Western blots showed loss of the corresponding proteins relative to mock treated cells (no sgRNA or Cas9). For seven days post-transfection, cells were assessed for the presence of the protein target via immunoblot analysis (GAPDH expression across the same timeframe was used for normalization).

 

multi-guide KO gel-1

Figure 5. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.

Resources

Application Note
Multi-guide RNA Improves CRISPR Efficiency by Generating Fragment Deletions
Learn how intelligently designed multiple gRNAs improve CRISPR knockout efficiency with single exon fragment deletions.
Learn More
E-book
Cell Engineering 101 eBook
Discover methods and insights into skillfully generating knockout or knock-in CRISPR edits in a wide-range of cell lines.
Learn More
flyer
Gene Knockout Kit: Elevate Your Guide Design Strategy
EditCo's Gene Knockout Kit takes the trial and error out of CRISPR knockouts by using a novel strategy of guide RNA design to knock out any human or mouse protein-coding gene.
Learn More
Quick Start Guide
Gene Knockout Kit Quick Start Guide
This quick-start guide can be used for EditCo's Gene Knockout Kit. In this guide, we include important information on how to store, quantify, and transfect your gRNA kit and analyze your knockout kit experiments.
Learn More
Overview

Find your targets faster with unparalleled CRISPR knockout efficiency and speed

  • High Knockout Efficiency: Multi-guide design that results in functional knockouts of your targets—reliable results,  guaranteed
  • Robust Quality Control: Consistently high quality and precision when plating provide you with reliable knockout results across your entire screen.
  • Unrivaled Delivery Speed: Faster turnaround time and consistent delivery means you get to your experiments quicker.
unnamed (83)

Screen Smarter Not Harder. Effective Knockout Strategy for Better CRISPR Screening.

Other loss-of-function screening libraries are often plagued with laborious preparation, data analysis, poor editing efficiency and low quality reagents that can be cytotoxic to more sensitive cell types such as primary cells. Our Arrayed CRISPR gRNA Libraries are different.

Powered by EditCo’s unique multi-guide design, our Arrayed CRISPR gRNA Libraries are comprised of SpCas9 sgRNAs targeting one gene per well in a multiwell plate format. The multi-guide design consists of up to three spatially coordinated SpCas9 sgRNAs that induce fragment deletions in an early single exon of a gene. Large fragment deletions in the target gene increased likelihood of functional knockout by not relying on random indel creation. Overall, this decreases the likelihood of false negatives, reliably knocking out any human or mouse protein-coding gene. 

Our libraries are ready-to-transfect, either complexed into RNPs or directly into a Cas9-expressing cell line avoiding long preparation protocols. The arrayed format avoids the need of messy next-generation sequencing (NGS) data deconvolution to interpret your screening results and allows to perform binary and multiparametric functional assays. Furthermore, you can analyze your CRISPR edits in seconds using Sanger sequencing with our CE tool. Our unique multi-guide design ensures you can genotype with one single reaction.

EC_library-workflow (1)

Figure 1. EditCo Arrayed Libraries Workflow. Incorporating smart guide design with automation coupled with our high-quality control, we plate only the best-quality sgRNA into each well with high precision enabling you to reliably knock out your targets in your screens with confidence, we guarantee it (excluding non-essential genes). Due to our optimized workflow, we can deliver your Arrayed CRISPR gRNA Libraries in as few as 7 days at unrivaled speed.

Whole Genome

Comprehensive Gene Coverage for Whole Human or Mouse Genomes

Powered by our unique multi-guide design, we offer both Whole Human and Whole Mouse Genome libraries. Our Whole Genome Libraries ensure the highest editing efficiencies, decreasing false negatives. Our libraries are also delivered ready-to-transfect, minimizing laborious preparation across over 20,000 gene targets.

Our Whole Human and Whole Mouse Genome libraries enable you to get the most of your target identification screen, but if you are more interested in studying your specific gene set or a specific pathway, check out our User-defined or Pathway Libraries.

Features

Species
  • Human
  • Mouse
Sizes
  • Human - 150 pmol to 10 nmol
  • Mouse - 30 pmol to 150 pmol
Plate Format
  • Human 96-well plate: Nunc Deepwell
  • Human 384-well plate: Nunc, ECHO PP, or ECHO LVD
  • Mouse 384-well plate: Nunc or ECHO PP

Deliverables

  • SpCas9 multi-guide plated library (up to 3 guides per well), dry
  • sgRNAs are modified: 2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases (modifications help resist degradation and prevent triggering intracellular immune responses)
  • Library plate layouts (.csv)
  • Multi-guide sgRNA sequences
  • Primer sequences for PCR and Sanger sequencing

Quotes and ordering 

If you need a quote to place your order, request a quote by clicking below.

 Contact Us

Pathway Libraries

Transform Your Target Discovery With Our Pathway Libraries

We offer 30+ Pathway Libraries including druggable, GPCRs, kinases, and immuno-oncology targets. These libraries are well-suited for target identification studies, providing a comprehensive gene set to begin your discovery process. Select Pathway libraries are shipped in as quickly as one week so you can begin your screen even sooner. Our Pathway Libraries are powered by our unique multi-guide strategy to create predictable knockouts with high-efficiency and reduce false negatives. See table below for a full list of available Pathway Libraries - contact us to order. If you are interested in more widespread coverage, check out our Whole Genome Libraries. If you are interested in a specific set of targets, check out our User-defined Libraries.

Contact our sales team for more information about our libraries including what in stock libraries are ready to ship!

Available Gene Pathways

Pathway Libraries List Number of Genes in Library

Apoptosis Pathway Library

1997

Autophagy Library

460

B-Cell Activation Pathway Library

242

Cell Adhesion Genes Library

1479

Cell Cycle Regulators Library

1251

Cell Surface Proteins Library

2738

Complete Druggable Library

8478

Kinase Library

1035

Cytokines and Chemokines Library

310

Cytoskeleton Genes Library

1745

Deubiquitinating Enzymes Library

117

DNA Repair Pathway Library

530

Epigenetic Regulators Library

826

Essential Genes Library

2553

Extracellular Matrix Genes Library

363

G-Protein Coupled Receptors Library

1046

Helicases Library

154

Immunology/Immuno-Oncology Library

3098

Ion Channels Library

685

JAK-STAT Pathway Library

194

Metabolic Activity Library

11432

Nuclear Hormone Receptors Library

192

p53 Pathway Library

319

Pattern Recognition Receptors and Signaling Pathways Library

303

Phosphatases Library

344

SARS-CoV-2 Druggable Interactome Library

63

SARS-CoV-2 Interactome Library

324

Secreted Proteins Library

5292

Serine Proteases Library

263

T-Cell Activation Pathway Library

524

Transcription Factors Library

2358

Tumor Suppressors Library

1055

Tyrosine Kinases Library

152

Ubiquitin Ligases Activity (E1, E2, E3) Library

1159

Ubiquitin Protein Ligases Library

336

 

Features

Species
  • Human
Sizes
  • Human - 150 pmol to 10 nmol
Plate Format
  • Human 96-well plate: Nunc Deepwell
  • Human 384-well plate: Nunc, ECHO PP, or ECHO LVD

Deliverables

  • SpCas9 multi-guide plated library (up to 3 guides per well), dry
  • sgRNAs are modified: 2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases (modifications help resist degradation and prevent triggering intracellular immune responses)
  • Library plate layouts (.csv)
  • Multi-guide sgRNA sequences provided for each gene target
  • Primer sequences for PCR and Sanger sequencing

Quotes and Ordering

If you need a quote to place your order, request a quote by clicking below.

 Contact Us

User-Defined

Confidently Knock Out Your Target Set of Genes

As part of our multi-guide knockout libraries, we also offer User-defined Libraries so you can choose your specific gene set from human and mouse genomes, well-suited for target validation studies. Human User-defined Libraries are shipped in as quickly as one week. Our User-defined Libraries are powered by our unique multi-guide strategy to create predictable knockouts with high-efficiency and reduce false negatives. All libraries arrive ready-to-transfect, eliminating laborious and time-intensive steps that alternative libraries require and can be used to screen in any human or mouse cell type, including primary cells. 

Features

Species
  • Human
  • Mouse
Sizes
  • Human - 150 pmol to 10 nmol
  • Mouse - 1500 pmol
Plate Format
  • Human 96-well plate: Nunc Deepwell
  • Human 384-well plate: Nunc, ECHO PP, or ECHO LVD
  • Mouse 96-well plate: Nunc Deepwell

Deliverables

  • SpCas9 multi-guide plated library (up to 3 guides per well), dry
  • sgRNAs are modified: 2' O-Methyl analog on first and last 3 bases; 3' phosphorothioate between first 3 and last 2 bases (modifications help resist degradation and prevent triggering intracellular immune responses)
  • SpCas9 proprietary EZ Scaffold
  • Library plate layouts (.csv)
  • Multi-guide sgRNA sequences provided for each target
  • Primer sequences for PCR and Sanger sequencing
  • User-selected add-on controls

Quotes and ordering 

If you need a quote to place your order, request a quote by clicking below.

  Contact Us

Data

Optimal Knockout Efficiency for High-throughput

How Does EditCo's unique guide design induce a fragment deletion?

EditCo’s multi-guide design is composed of up to 3 sgRNAs targeting an early single exon of a gene of interest. The guides are spatially coordinated to induce a guided repair that results in fragment deletions. The guide spacing was also designed to guarantee effective analysis of your CRISPR edits with one single reaction and in seconds using Sanger sequencing with our free unique bioinformatics tool, Inference of CRISPR Edits (ICE).

Multi-guide Schematic

 

 

Consistent and Reliable Knockouts

Libraries Multi-guide Scatter

Figure 3. Better knockout efficiency was found across 32 genetic targets assessed. EditCo’s multi-guide design consistently results in high Knockout (KO) Scores across 32 genetic targets assessed compared to strategies that rely on individual gRNAs. gRNAs displayed 29.2% better median knockout efficiency when introduced in a multi-guide format (89.9% KO score) relative to an individual sgRNA format (69.6% KO Score).

 

Achieve Robust Genotype and Phenotype Screening Results

Viability Screening

Figure 2. Across 77 genes, arrayed multi-guide library resulted in an average Knockout Score of 75%. Using our multi-guide algorithm, up to 3 modified sgRNA per target (across 86 genes) were transfected into U2OS-Cas9 expressing cell line using nucleofection. This resulted in an average Knockout Score of 75% for 77 of the genes. Nine of the 86 genes failed due to sequencing errors.

 

Multi-guide KO Gel

Figure 3. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.

EditCo Libraries Enables High Editing Efficiencies in a Large-scale Primary Cell Screen

Large Screen Efficiency

Figure 4. Screening showed a 97% median editing efficiency using across 280 loci. Multi-guide sgRNA was transfected into primary dendritic cells using nucleofection. Editing efficiency was assessed via NGS across 280 loci. Data courtesy of Weissman Lab, UCSF.

Resources

Application Note
Multi-guide RNA Improves CRISPR Efficiency by Generating Fragment Deletions
Learn how intelligently designed multiple gRNAs improve CRISPR knockout efficiency with single exon fragment deletions.
Learn More
Flyer
Target Discovery: The Path to Screening with Confidence
EditCo offers CRISPR solutions from start-to-finish in your loss-of-function screening pipeline for more accurate, productive, and reproducible results.
Learn More
Quick Start Guide
Arrayed CRISPR gRNA Libraries Quick Start Guide
EditCo’s human and mouse screening libraries utilize multi-guide gRNA that has been strategically designed to knock out your human or mouse protein-coding gene of interest. This quick-start guide provides instructions on how to prepare, use, store and quantify your gRNA library.
Learn More
EditCo's Automated CRISPR Platform

Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.

reagent_workcell
Icon_Pools_Trans
Leveraging a sophisticated infrastructure with integration between bioinformatics, software, and automated platforms
Learn More
Icon_TCell_Trans
Automating biology and synergistic disciplines for streamlined cell editing and cell culture workflows
Learn More
Icon_iPSC_Trans
Delivering experiment-ready edited cells for your next discoveries through a robust and cohesive ecosystem
Learn More
Lorem Overline

Testimonials consectitur amet sed

Lorem Button Text
Congue fermentum eros leo commodo. Integer vitae vitae ac amet. Vitae sed lectus vel lacus adipiscing nulla facilisis mauris porttitor. Viverra elementum odio vitae id.
placeholder-56x56
Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
Viverra aliquam volutpat interdum quisque vel mattis. Malesuada aliquam egestas pharetra a tempus ullamcorper egestas.
placeholder-56x56
Amanda Peyton, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute
Nibh luctus pulvinar sagittis faucibus commodo vitae purus. Ullamcorper dictum.
placeholder-56x56
Joseph James, Ph. D.
Principal Investigator, Boston Children's Hospital
Congue fermentum eros leo commodo. Integer vitae vitae ac amet. Vitae sed lectus vel lacus adipiscing nulla facilisis mauris porttitor. Viverra elementum odio vitae id.
placeholder-56x56
Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
Congue fermentum eros leo commodo. Integer vitae vitae ac amet. Vitae sed lectus vel lacus adipiscing nulla facilisis mauris porttitor. Viverra elementum odio vitae id.
placeholder-56x56
Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
Congue fermentum eros leo commodo. Integer vitae vitae ac amet. Vitae sed lectus vel lacus adipiscing nulla facilisis mauris porttitor. Viverra elementum odio vitae id.
placeholder-56x56
Albert Rose, Ph. D.
Principal Investigator, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School
Lorem Button Text

Lectus Pluristyx eu vel leo lectus mi. Viverra cursus neque eget proin.

Pellentesque nec tempus ultrices purus amet adipiscing aliquam. Posuere imperdiet adipiscing non blandit non. Sed eros vel sodales magna sem tincidunt orci risus. Molestie hendrerit cursus faucibus ac neque eget turpis. Arcu leo risus iaculis mi. Tortor bibendum sed cras pretium leo.

0+

Edits Performed

0+

Edits Performed

0+

Edits Performed
Lorem Overline

Lorem Ipsum Dolor Heading

Etiam sollicitudin, ipsum eu pulvinar rutrum, tellus ipsum laoreet sapien, quis venenatis ante odio sit amet eros. Duis arcu tortor, suscipit eget, imperdiet nec, imperdiet iaculis, ipsum. Maecenas nec odio et ante tincidunt tempus. Donec elit libero, sodales nec, volutpat a, suscipit non, turpis. Quisque id mi.

Lorem Button Text
Lorem Overline

Resources aliquam procte

Lorem Button Text
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
placeholder-280x160
Lorem overline
Ultrices nullam dictumst erat molestie massa a amet pulvinar bibendum lacus.
Tortor consectetur viverra malesuada dignissim duis quis. Morbi purus in velit lacus diam odio sed. Sed nisl arcu amet ut.
Read More
Lorem Button Text

Reach out to our experts to find out what EditCo can do for you