Poster: HiBiT-Tagged CRISPR Cell Line Engineering: Enabling Antibody-Free Protein Quantification and Targeted Degradation Analysis in Cancer Research

Conclusions

• HiBiT tagging enables rapid, antibody-free quantification of endogenous protein levels in engineered cancer cell lines.
• CRISPR-mediated knock-in of HiBiT is compatible with a wide range of gene targets and cell types, including hard-to-edit immortalized cancer models.
• The resulting cell lines support real-time profiling of protein degradation, stability, and drug engagement, providing a high-throughput alternative to traditional immunoassays.
• Integration of NGS verification ensures editing precision and consistency, making the platform suitable for mechanistic studies and functional screening.
• This approach offers academic researchers a scalable and reproducible system for exploring cancer biology with enhanced temporal resolution and data quality.

Discover more in our Editorial series:

HiBiT-Tagged Knock-In Cells: The End of the Era of Protein Overexpression

Choosing the Right Protein Tag for Your Experiment