Knockout Immortalized Cell Lines
EGFR Knockout in PC9 Cell Line (Human)
Fast, Reliable CRISPR Knockout Pools and Clones. Consistent knockouts you can trust leveraging our XDel multi-guide designs with 98% average editing efficiency. NGS-verified with QC report, assay ready cells shipped in as fast as 2 weeks. *Please contact your EditCo representative for academic pricing.
EGFR
ENSG00000146648The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]
- Guides designed
- 3
- Cuts canonical
- Yes
- Coding seq. cut
- 90%–93%
- CCDS cut
- 7 of 7
- Earliest exon cut
- Exon 3
- Off-target genes
- None
Cancer cell line · Male · 45Y · lymph node
Popular targets by cell line
Data
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.