Knock-in Immortalized Cells

CRISPR-edited Immortalized Knock-in Cells

Precision CRISPR knock-in immortalized cell lines — pools or clones with reliable, high-efficiency edits, delivered assay-ready for your experiments.

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Overview

Precision CRISPR Knock-ins for Streamlined and Efficient Success

High success rate

Our automated optimization conquers cell line variability, identifying the perfect transfection conditions for maximum editing efficiency.

Minimize off-targets

With stringent guide design criteria and RNP-based delivery, EditCo ensures precise edits with minimal off-target effects.

Accessibility

Expand your research without overspending. Model more genes or variants in more cell lines and see how much farther your budget can go.

Access CRISPR Cell Lines Without Sacrificing Time

EditCo’s Knock-in Immortalized Cells are your quickest path to integrating engineered cells into your research. Whether modeling disease with SNVs or tagging proteins for in-depth studies, DIY CRISPR projects often come with unforeseen challenges and delays, typically requiring several experimental repeats and adding months to your timeline. EditCo’s advanced automated transfection optimization and top-tier reagents maximize editing efficiency, guaranteeing a successful CRISPR edit. Unlock your research potential with EditCo’s Knock-in Immortalized Cells and accelerate your path to discovery.

EditCo’s Automated Immortalized Cell Line Editing Process

Quantify Your Target Protein: Tag your protein of interest for easy purification and quantification.
Generate a Disease Model: Introduce or correct a disease-causing mutation to study a disease phenotype.
Measure Gene Regulation: Generate a reporter cell line using a promoter tag for gene regulation studies.
Elucidate Protein-Protein Interactions: Identify the interactome of protein through the incorporation of affinity tags.

Single-guide RNA Knock-in Cell Pools

Economical High-throughput CRISPR Optimization for Knock-in Immortalized Cells

EditCo’s Knock-in Immortalized Cell Pools provide a cost-effective solution, offering a mixed population of transfected cells with both edited and unedited cells. Harness the power of EditCo’s high-throughput CRISPR optimization and advanced knock-in strategies while managing clonal isolation on your terms. For those looking to save hands-on time and effort, explore our Knock-in Immortalized Cell Clones, where we handle the isolation process for you.

Features

Cell Source	EditCo supplied (standard) Customer supplied
Available Edits	SNV Tag Large insertion
CRISPR Design	Synthetic modified sgRNA (standard) DNA HDR template (standard)

Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided

Deliverables

Periodic updates on your order's progress
Mock-transfected cell pools (2 vials)
Sequence of synthetic sgRNA and HDR template used
Primer sequences used for NGS sequencing
NGS sequencing analysis report for each edited pool after expansion
Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC

Successful Knock-ins in Any Cell Line

EditCo’s CRISPR Knock-in Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.

Successful knock-in editing across immortalized cell lines — **Figure 1. Successful knock-in editing, even in hard-to-edit cell lines, enabled by our automated transfection optimization protocol.** gRNAs and ssODNs were designed to induce a 33nt knock-in and then transfected into the 9 different immortalized cell lines indicated. Post recovery, knock-in editing efficiencies were measured in the mixed population of edited and unedited cells using EditCo’s ICE Knock-in analysis tool. The cells are then either used directly for assays or isolated into clonal populations.

**Figure 2. High knock-in efficiencies across numerous loci.** sgRNA and ssODNs were designed to induce a 9-nt knock-in at numerous different loci, then transfected into multiple different cell types. Post cell recovery, knock-in editing efficiencies were assessed using EditCo’s ICE Knock-in analysis tool. The values displayed above represent the percentage of each total population of cells that have the desired knock-in edit present.

Single-guide RNA Knock-in Cell Clones

Precise Knock-in Immortalized Cell Clones Done for You

EditCo’s Knock-in Immortalized Cell Clones offer a clonal population of cells with sequence-verified knock-ins. Whether you need to endogenously tag a protein or alter an SNV, our Knock-in Immortalized Clones provide the simplest path to achieving precise, high-quality edits in your lab. Unlike our pool format, these clones undergo EditCo’s automated isolation process, ensuring you receive a population derived from a single cell with your desired knock-in.

Features

Cell Source	EditCo supplied (standard) Customer supplied
Genetic Modifications	SNV Tag Large insertion
CRISPR Design	Synthetic modified sgRNA (standard) DNA HDR template (standard)
Add-Ons	Additional clones Additional vials Intermediate pools QC: Western blot QC: Flow cytometry QC: Whole genome sequencing

Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided

Deliverables

Periodic milestone updates as we make progress on your order
2 individual edited clones (2 vials of each clone)
Mock-transfected cell pools (2 vials)
Sequence of synthetic sgRNA and HDR template used
Primer sequences used for NGS sequencing
NGS sequencing analysis report for each edited pool after expansion
Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC