Product OverviewOrder KitContact Sales
CRISPR Reagents

Gene Knockout Kits (GKO Kits)

U2AF1 Gene Knockout Kit (Human)

Configure your kit

Bulk Upload

$409.00

Need a quote? Add to cart and download one instantly at checkout — to reference on a purchase order, submit for budget approval, or keep for your records. No need to contact sales.

The same high performing XDel multi-guide design that powers our knockout cells delivered in as fast as 2 days for human genes. Knock out any human or mouse protein-coding gene with >80% efficiency.

Learn More About Gene Knockout KitsDownload Quick Start GuideExplore More Data in XDel Application Note

Gene

U2AF1

ENSG00000160201
Summary
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Gene Ontology
Molecular Function6
Biological Process3
Cellular Component7
Popular knockout targets

Data

Gene Knockout Kits Provide Superior Editing Efficiency in iPSC, Immortalized, and Primary Cells and Consistent Protein Depletion Using the XDel Design

Figure 1. A) On-target editing efficiency of XDel multiple guide design (MGKO) (pink bars) vs their 3 respective single-guide RNAs (blue bars) across 7 endogenous target loci transfected in two immortalized (IMM) and two iPSC cell lines. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis. B) Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.

Explore More Data

About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.