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CRISPR Reagents

Gene Knockout Kits (GKO Kits)

MED4 Gene Knockout Kit (Human)

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The same high performing XDel multi-guide design that powers our knockout cells delivered in as fast as 2 days for human genes. Knock out any human or mouse protein-coding gene with >80% efficiency.

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Gene

MED4

ENSG00000136146
Summary
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Gene Ontology
Molecular Function5
Biological Process6
Cellular Component5
Popular knockout targets

Data

Gene Knockout Kits Provide Superior Editing Efficiency in iPSC, Immortalized, and Primary Cells and Consistent Protein Depletion Using the XDel Design

Figure 1. A) On-target editing efficiency of XDel multiple guide design (MGKO) (pink bars) vs their 3 respective single-guide RNAs (blue bars) across 7 endogenous target loci transfected in two immortalized (IMM) and two iPSC cell lines. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo’s proprietary software analysis. B) Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.

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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.