Immortalized Cells: Enhance Your Research with EditCo's Reliable CRISPR-edited Cell Lines for High-Quality Reproducible Results
EditCo's Immortalized Cells offer robust and reproducible models for gene function studies, drug development, and therapeutic research. Delivering consistent performance and reliability, these cells accelerate scientific discovery by democratizing access to high-quality engineered cell lines.
Reliable, Reproducible Models for Accelerated Research
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Invest in results: Spend your time running experiments, not generating your CRISPR knockouts.
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Finish Experiments Faster: We’ll handle the CRISPR editing so you can run your assays sooner.
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Assay with confidence: Trust your downstream assay results with EditCo’s incredibly high knockout efficiencies.
Accelerate Your Research with EditCo’s Knockout CRISPR Cell Lines
Creating genetically modified CRISPR knockout cells traditionally takes months and involves a high risk of failure. Our benchmark survey reveals that researchers invest an average of 61 hours in hands-on work before even isolating a clone, with 19.1 hours spent on optimization and 13.7 hours on transfection. EditCo's advanced automated optimization and transfection processes eliminate these time-consuming steps, enabling you to reclaim precious research hours. Transform your research workflow with EditCo’s knockout cells, and focus on achieving groundbreaking discoveries faster.
Accelerate Your Discoveries with CRISPR Knockout Cells
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- Single, double, or triple knockout
CRISPR Design
- Synthetic modified sgRNA (standard)
Add-Ons
- Additional clones
- Additional vials
- Intermediate pools
Deliverables
- Regular updates on your order's progress
- 2 independent clones with the required knockout (2 vials of each clone with 5x10⁵ cells/vial)
- Control-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA used
- Primer sequences used for PCR and Sanger Sequencing
- ICE Sanger sequencing analysis reports for each edited clone after expansion
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Rapid, High-Efficiency CRISPR Knockout
With EditCo’s Knockout Immortalized Cell Pools, we handle the complex CRISPR editing, ensuring a 50 percent or better knockout of your target gene. These pools offer a mixed population of edited and unedited cells, ready for immediate assay validation or further clonal isolation.
Focus on making discoveries, not on CRISPR optimization. Experience the benefits of CRISPR-engineered cells with just one click.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- Single, double, or triple knockout
CRISPR Design
- Synthetic modified sgRNA (standard)
Deliverables
- Regular updates on your order's progress
- Edited cell pools (2 vials with 5x10⁵ cells/vial)
- Control-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA used
- Primer sequences used for PCR and Sanger Sequencing
- ICE Sanger sequencing analysis reports for each edited clone after expansion
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Higher Knockout Efficiency and Functionality
200 Ways We Optimize CRISPR to Achieve High Editing Across Cell Lines
No two cell lines are the same, so there’s no single best CRISPR protocol for all cells. We perform a rapid high-throughput optimization for every cell line to achieve high-efficiency knockouts in cell lines across numerous tissue types.
Figure 1. EditCo’s 200-point optimization has been performed in over 100 human cell lines across 15 tissue types to identify the best parameters that yield the highest editing efficiency for each cell line. High editing efficiencies were observed in easy, moderate, and challenging cell and tissue types. Example cell lines (noted by * and color) that were relatively b) easy (red), c) moderate (green), and d) challenging (blue) are shown.
Efficient Generation of Knockout Clones with Functional Outcomes
EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.
Figure 2. Optimized gRNA design for the highest efficiency knockouts. EditCo’s 200-point optimization has been performed in over 100 human cell lines across 15 tissue types to identify the best parameters that yield the highest editing efficiency for each cell line. High editing efficiencies were observed in easy, moderate, and challenging cell and tissue types. Example cell lines (noted by * and color) that were relatively b) easy (red), c) moderate (green), and d) challenging (blue) are shown.
Validate your Assays with up to 95% Protein Knockout
EditCo’s Knockout Cell Pool gives you the protein knockout you need in the cell line you want, enabling you to either assay pools directly or quickly move to isolate single cell clones.
Figure 3. EditCo’s Knockout Cell Pool achieved 95 percent protein knockout without clonal expansion. A cell pool with a surface protein knocked out was generated in U2OS cells with a Knockout Score of 87 percent (left panel, which is the percentage of indels that either introduce a frameshift mutation or are larger than 21 nucleotides, analyzed by ICE). Levels of this protein were analyzed by flow cytometry (blue) and were reduced by 95 percent relative to wild type (green, right panel). Negative control samples were stained with an isotype control antibody (purple). Data provided by Eurofins-DiscoverX.
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Precision CRISPR Knock-ins for Streamlined and Efficient Success
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High success rate: Our automated optimization conquers cell line variability, identifying the perfect transfection conditions for maximum editing efficiency.
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Minimize off-targets: With stringent guide design criteria and RNP-based delivery, EditCo ensures precise edits with minimal off-target effects.
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Accessibility: Expand your research without overspending. Model more genes or variants in more cell lines and see how much farther your budget can go.
Access CRISPR Cell Lines Without Sacrificing Time
EditCo’s Knock-in Immortalized Cells are your quickest path to integrating engineered cells into your research. Whether modeling disease with SNVs or tagging proteins for in-depth studies, DIY CRISPR projects often come with unforeseen challenges and delays, typically requiring several experimental repeats and adding months to your timeline. EditCo’s advanced automated transfection optimization and top-tier reagents maximize editing efficiency, guaranteeing a successful CRISPR edit.
Unlock your research potential with EditCo’s Knock-in Immortalized Cells and accelerate your path to discovery.
Precise Knock-in Immortalized Cell Clones Done for You
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Genetic Modifications
- SNV
- Tag
- Large insertion
CRISPR Design
- Synthetic modified sgRNA (standard)
- DNA HDR template (standard)
Add-Ons
- Additional clones
- Additional vials
- Intermediate pools
Deliverables
- Periodic milestone updates as we make progress on your order
- 2 individual edited clones (2 vials of each clone)
- Mock-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA and HDR template used
- Primer sequences used for PCR and Sanger Sequencing
- Sequencing analysis reports for each edited clone after expansion
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Economical High-throughput CRISPR Optimization for Knock-in Immortalized Cells
EditCo’s Knock-in Immortalized Cell Pools provide a cost-effective solution, offering a mixed population of transfected cells with both edited and unedited cells. Harness the power of EditCo’s high-throughput CRISPR optimization and advanced knock-in strategies while managing clonal isolation on your terms. For those looking to save hands-on time and effort, explore our Knock-in Immortalized Cell Clones, where we handle the isolation process for you.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- SNV
- Tag
- Large insertion
CRISPR Design
- Synthetic modified sgRNA (standard)
- DNA HDR template (standard)
Deliverables
- Periodic updates on your order's progress
- Mock-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA and HDR template used
- Primer sequences used for PCR and Sanger Sequencing
- Sequencing analysis reports for each edited pool after expansion
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Precision Knock-in Clones and Pools
Successful Knock-ins in Any Cell Line
EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.
Figure 1. Successful knock-in editing, even in hard-to-edit cell lines, enabled by our automated transfection optimization protocol. gRNAs and ssODNs were designed to induce a 33nt knock-in and then transfected into the 9 different immortalized cell lines indicated. Post recovery, knock-in editing efficiencies were measured in the mixed population of edited and unedited cells using EditCo’s ICE Knock-in analysis tool. The cells are then either used directly for assays or isolated into clonal populations.
Figure 2. High knock-in efficiencies across numerous loci. sgRNA and ssODNs were designed to induce a 9-nt knock-in at numerous different loci, then transfected into multiple different cell types. Post cell recovery, knock-in editing efficiencies were assessed using EditCo’s ICE Knock-in analysis tool. The values displayed above represent the percentage of each total population of cells that have the desired knock-in edit present.
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Accelerate Your Research with Express Cell Pools
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Faster data: Get your Knockout pool in 2 weeks or less. Focus on your assay and generate data faster.
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Confident results: 80 percent editing efficiency guaranteed (except for common essential genes).
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Flexibility: Choose from EditCo ATCC supplied cells or onboard your cell line.
Generate Assay Results with Speed and Confidence
Express Cell Pools are highly knockout-edited pools of cells consisting of a heterogeneous population of edited and unedited cells for your gene of interest in human and mouse immortalized cell lines, generated in 2 weeks or sooner.
Whether you are leveraging CRISPR for validating targets for drug discovery or modeling a biological process for the investigation of a novel gene of interest, failed experiments lead to a loss of time and resources. EditCo’s Express Cell Pools are the solution for obtaining quick and reliable functional data while reducing the risk for false negatives in your critical assays.
Express Cell Pools workflow includes the use of our unique multi-guide design, transfection, genotyping, and sequencing analysis. Choose from either EditCo supplied cell lines banked from our high-quality cell supplier ATCC, or onboard your cells.
Alternatively, if you would like to use your sgRNA sequence, Express Custom Cell Pools are also available. These allow user-defined sgRNA sequences to specifically target the transcript or genomic region of interest. Please contact us for further information.
Rapid, High-Efficiency CRISPR Knockout
Focus on Your Assay and Entrust CRISPR to US
Our Express Cell Pools are guaranteed to have at least 80% editing efficiency. Now available using EditCo ATCC supplied cell lines or onboard your cells.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Single knockout
CRISPR Design
- Multi-guide synthetic modified sgRNA (standard)
Deliverables
- Regular updates on your order's progress
- 2 vials of edited cell pools (a heterogeneous population of edited and unedited cells) with >300,000 cells/vial
- Control-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA used
- Primer sequences used for PCR and Sanger Sequencing
- ICE Sanger sequencing analysis reports for each edited clone after expansion
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Use a Wide Variety of ATCC Cells Lines or Onboard Your Own
Available ATCC Cell Lines for Express Knockout Cell Pools
Cell Line | Tissue Type | Cellular Morphology | Disease | Species |
K562 | Blood | Lymphoblast | Chronic Myelogenous Leukemia | human |
Raji | Blood | Lymphoblast | Burkitts lymphoma | human |
REH | Blood | Lymphoblast | Acute lymphocytic leukemia | human |
HL60 | Blood | Lymphoblast | Acute promyelocytic leukemia | human |
MM.1R | Blood - B Cell | Lymphoblast | Multiple Myeloma | human |
RPMI8226 | Blood - B Cell | Lymphoblast | Plasmacytoma | human |
SU-DHL-4 | Blood - B Cell | Lymphoblast | B cell leukemia | human |
U266B1 | Blood - B Cell | Lymphoblast | Myeloma | human |
HEL 92.1.7 | Blood - Erythroblast | Lymphoblast | Erythroleukemia | human |
JURKAT | Blood - Macrophage | T-cell lymphocyte | Acute T cell leukemia | human |
MOLT4 | Blood - Macrophage | Macrophage; monocyte | Acute lymphoblastic leukemia | human |
RAW 264.7 | Blood - Macrophage | Macrophage; monocyte | Abelson murine leukemia virus-induced tumor | mouse |
THP1 | Blood - Macrophage | Macrophage; monocyte | Acute monocytic leukemia | human |
HMC3 | Blood - Microglia | Macrophage; Microglia | None; SV40-dependent immortalization of a human fetal brain-derived primary microglia culture. | human |
U2OS | Bone | Epithelial | Osteosarcoma | human |
Daoy | Brain | Polygonal | Desmoplastic Cerebellar Medulloblastoma | human |
H4 | Brain | Epithelial | Neuroglioma | human |
Neuro-2a | Brain | Neuronal | Neuroblastoma | mouse |
U87MG | Brain | Epithelial | Glioblastoma | human |
HELA | Cervix | Epithelial | Adenocarcinoma | human |
CT26.WT | Colon | Fibroblast | Carcinoma | mouse |
DLD1 | Colon | Epithelial | Adenocarcinoma; Colorectal; Dukes' type C | human |
HCT116 | Colon | Epithelial | Carcinoma | human |
LOVO | Colon | Epithelial | Adenocarcinoma; Colorectal; Dukes' type C, grade IV | human |
SW620 | Colon | Epithelial | Adenocarcinoma; Colorectal; Dukes' type C | human |
T84 | Colon | Epithelial | Carcinoma; Colorectal | human |
HT1080 | Connective Tissue | Epithelial | Fibrosarcoma | human |
786-O | Kidney | Epithelial | Adenocarcinoma; Renal Cell | human |
HEK293 | Kidney | Epithelial | None; embryonic isolation | human |
HEP3B | Liver | Epithelial | Carcinoma; Hepatocellular | human |
Hepa-1c1c7 | Liver | Epithelial | Hepatoma | mouse |
HEPG2 | Liver | Epithelial | Carcinoma; Hepatocellular | human |
A549 | Lung | Epithelial | Carcinoma | human |
LL/2 (LLC1) | Lung | Epithelial or rounded. | Lewis Lung Carcinoma | mouse |
4T1 | Mammary | Epithelial | Mimics human breast cancer; Stage IV | mouse |
AU565 | Mammary | Epithelial | Adenocarcinoma | human |
EMT6 | Mammary | Epithelial | Carcinoma | mouse |
ASPC1 | Pancreas | Ascite | Adenocarcinoma | human |
BXPC3 | Pancreas | Epithelial | Adenocarcinoma | human |
PANC1 | Pancreas | Epithelial | Epithelioid Carcinoma | human |
22RV1 | Prostate | Epithelial | Carcinoma | human |
DU145 | Prostate | Epithelial | Carcinoma | human |
PC3 | Prostate | Epithelial | Adenocarcinoma; Grade IV | human |
A375 | Skin | Epithelial | Malignant Melanoma | human |
B16-F10 | Skin | Mixture of spindle-shaped and epithelial-like cells | Melanoma | mouse |
If the cell line you are interested in is not available in above table, learn about onboarding your own below.
EditCo's Express Cell Pool Service for Your Unique Cell Lines
Whether you're in need of human tumor cell lines from esteemed sources like the National Cancer Institute, your trusted workhorse cell line, or a previously edited line, EditCo is dedicated to simplifying your research journey. Bid farewell to the complexities of cell optimization and gene editing–simply onboard your cells, and let us take care of the cell editing with our state-of-the-art Express Cell platform.
Our User-Supplied Express Cell Knockout Pools involve a straightforward two-step process:
- Cell Banking: Your supplied cell line undergoes EditCo's proprietary banking process, ensuring high quality through quality control (QC) measures. This includes a strict QC to ensure we can deliver high-quality edited cells in 2 weeks or less after the cell banking is complete. A detailed QC report is provided with all the parameters evaluated.
- Cell Editing: Once successfully banked, up to 20 edits can be generated per banking cycle. You have the flexibility to choose when edits are performed, with no minimum requirement. Our edited cell knockout pools guarantee at least 80 percent editing efficiency. Edited cells undergo the same rigorous QC process as our standard Express Cell platform. Each edit includes 2 vials of edited cell pools and 2 vials of wild-type cells, each containing over 300,000 cells per vial.
Key Benefits:
- Flexibility: Perform up to 20 edits from a single banking cycle.
- Fast turnaround: Receive edited cell pools (>300,000 cells/vial) in 2 weeks or less.
- High efficiency: We guarantee 80 percent editing efficiency for all knockout pools.
- Quality assurance: We perform stringent QC processes for banking and editing.
*Cell lines must meet the Express Platform growth conditions: < 72 hrs doubling time, no coating or shaking required, no complex media requirements, ability to grow at 37 C, 5% CO2, and BSL-2 safety only.
The Highest-Efficiency Knockout Strategy
Hundreds of Diverse and Efficient Knockouts Generated in Under 2 Weeks
Figure 1. Express Cell Pools generated across 355 genes and 36 different cell lines in just 12 days. Using EditCo’s multi-guide designs, up to 3 modified gRNAs per target were conjugated with SpCas9, and RNPs were transfected into cells. On average, Express Knockout Cell Pools were generated in 12 days and achieved >80 percent Gene Knockout (Knockout Score percentage).
Sustained Gene Knockout Across Cell Passages
Figure 2. High editing efficiency is sustained through multiple cell passages. Using EditCo’s multi-guide designs, up to 3 modified gRNA per target (across 6 genes) were conjugated with SpCas9, and RNPs were transfected into U2OS cells. Editing efficiencies were measured at different time points post-transfection and up to 4 passages. The median editing efficiency is >85 percent for each of the targets.
Cell Viability Is Maintained Across Passages
Figure 3. Cell viability is maintained through multiple cell passages in U2OS cells. Viability was taken using Celigo. Please note that CDK9 is a common essential gene, hence the lower viability and cell number observed along the passages.
Our Unique Guide Design Powers Functional Knockouts and Sustained Protein Depletion
Figure 4. The multi-guide approach consists of 3 gRNAs spatially coordinated to create a fragement deletion in a single exon. Targeting a single early exon of a gene induces multiple concurrent double-strand breaks in the target sequence of a gene. This results in disruptive one or more >21 bp fragment deletions. This approach is associated with higher frequencies of knockout alleles in edited pools but reduced occurrence of off-target edits as compared to single-guide approaches.
Figure 5. Edited cell pools utilizing the multi-guide technology shows persistent protein depletion. This enables direct and reliable use of these cell pools for functional assays. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.
Resources
Skip the Setup and Get Screening with Knockout Cell Libraries
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Faster results: Proceed directly to your screen and free up bandwidth, resources, and time.
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Scale easily: Remove limitations on your project scope, and increase your experimental scale.
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Greater certainty: Trust your screening results and confidently advance your research project.
Validate Drug Targets with Speed and Confidence
The high-throughput arrayed CRISPR screening format has emerged as a popular approach for target discovery because the results are amenable to straightforward interpretation without requiring labor-intensive data deconvolution steps and compatibility with a wide assortment of downstream assays. However, this approach has historically remained underutilized due to lack of infrastructure, challenges associated with transfection optimization, and/or limited bandwidth.
EditCo’s Engineered Cell Libraries lower these barriers to broaden the accessibility of CRISPR as a drug target discovery tool.
Engineered Cell Libraries are custom panels of edited immortalized or iPS cell pools in an arrayed format suitable for direct application in functional assays. EditCo's smart multi-guide design strategy enables editing efficiencies typically higher than 90 percent. Combined with the power and cell engineering capabilities high-throughput editing platform, you can generate libraries at any experimental scale, start your screen quickly, and confidently select the best hits.
Streamlined Intake and Optimized Process Yield Predictable Knockouts
The Engineered Cell Libraries workflow includes multi-guide design, high-throughput transfection, knockout pool generation, genotyping, and sequencing analysis.
What You Can Do
Everything You Need to Get Screening
Focus on Your Assay and Entrust CRISPR to us.
An Engineered Cell Library is a custom array of knockout immortalized or iPS cell pools aliquoted in tubes in a 96-well format, with each pool provided in duplicate. Each knockout cell pool in the library has been engineered using Synthego’s proprietary multi-guide technology to maximize the disruption of a specified gene target, allowing for direct application in functional assays. These Knockout Cell Libraries are provided with accompanying QC reports that provide insights regarding editing efficiencies, cell viability, and more.
Learn more about our Knockout iPSCs.
Features
Cell Type
- Immortalized human cell lines
- Immortalized mouse cell lines
- Human induced pluripotent stem cells
Cell Source
- EditCo supplied
- Customer supplied
Available Edits
- Knockout
CRISPR Design
- Multi-guide synthetic modified sgRNA (standard)
Deliverables
- Regular updates on your order's progress
- User-specified knockout cell pools per library (2 vials of each pool with ~20,000 cells/tube, 20 minimum order)
- 5 control pools per rack: 2 mock WT, 2 multi-guide KO, 1 single-guide KO
- Sequence of synthetic sgRNA used
- Primer sequences used for PCR and Sanger Sequencing
- ICE Sanger sequencing analysis reports
- Comprehensive QC report that includes mycoplasma test (positive/negative) and passage number
Engineered Cell Libraries Demonstrate High Editing Efficiencies Across Cell Types
Engineered Cell Libraries provide a set of CRISPR-edited cell pools that can be used directly in functional assays for a wide array of different screening and discovery applications. Editing efficiencies for each pool are assessed by sequencing and ICE analysis to inform the interpretation of assay results.
Knockouts in Human Immortalized Cell Line
Figure 1. A library of knockout cell pools corresponding to 42 gene targets was generated via high-throughput editing in human cells. Data from the experiment are shown above. An Engineered Cell Library with 42 gene targets was generated via high-throughput editing of an immortalized-adherent human cell line, A375, using multi-guide technology. The data demonstrate both indel frequencies and Knockout Scores. Of the 84 percent of pools successfully sequenced and analyzed, an average indel frequency of 99 percent and an average Knockout Score of 98 percent were observed.
Knockouts in Mouse Immortalized Cell Line
Figure 2. A library of knockout cell pools corresponding to 192 gene targets was generated via high-throughput editing in mouse cells. The data demonstrate both indel frequencies and Knockout Scores. Among the 80 percent of pools successfully analyzed, the average indel frequency was 89 percent, while the average Knockout Score was 84 percent.
Our Unique Guide Design Powers Functional Knockouts and Sustained Protein Depletion
The multi-guide approach consists of up to 3 gRNAs (green bars) spatially coordinated that target a single early exon of a gene and induce multiple concurrent double-strand breaks in the target sequence. This results in disruptive one or more >21 bp fragment deletions. This approach is associated with higher frequencies of knockout alleles in edited pools but reduced occurrence of off-target edits as compared to single-guide approaches.
Figure 3. The multi-guide approach consists of 3 gRNAs spatially coordinated to create a fragement deletion in a single exon. Targeting a single early exon of a gene induces multiple concurrent double-strand breaks in the target sequence of a gene. This results in disruptive one or more >21 bp fragment deletions. This approach is associated with higher frequencies of knockout alleles in edited pools but reduced occurrence of off-target edits as compared to single-guide approaches.
Figure 4. Edited cell pools utilizing the multi-guide technology shows persistent protein depletion. This enables direct and reliable use of these cell pools for functional assays. Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide gRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting gRNA.
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Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
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